The Field Of Cosmetics And The Treatment Of Injuries,...
Today, the field of cosmetics and the treatment of injuries, skin, and hair ailments have gained the
attention of doctors all over the world. While multiple treatment methods have been examined,
preference remains for the safest and the most effective method. Recently, treatment with PRP has
been established as an effective medical treatment in many fields, such as oral surgery, neurosurgery,
cosmetic surgery, sports medicine, and orthopedics. Platelets are one of the essential components
found in blood, known best for their importance in coagulation. They contain proteins known as
growth factors that play an essential role in healing damaged tissue (Lubkowska et al., 2012). The
normal range of platelets in healthy human blood ranges from ... Show more content on
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Despite these variations, all protocols follow the same sequence: blood sample collection, a soft spin
for separating blood components, followed by a hard spin for concentrating the platelets, in the
presence or absence of anticoagulants, and growth factor activators.
Kececi, Ozsu, and Bilgir (2014) examined the effect of the number of spins on the platelet recovery
during centrifugation. Both single and double centrifugation methods were used to prepare the PRP.
Two sets of blood samples were drawn from 10 adult New Zealand rabbits. The blood samples were
added into 10% sodium citrate and centrifuged; The first group was single centrifuged at 160 G for 6
minutes at room temperature, while the second group was double centrifuged, the first spin was at 160
G for 20 minutes at room temperature, followed by the second spin at 400 G for 15 minutes. Average
platelet amount collected from the second group (1,986,875 ± 685,020/μl) was greater than the first
group (781,875 ± 217,693/μl). According to Amable et al. (2013) different centrifugal forces have
been applied to obtain pure PRP from the blood samples obtained of healthy donors (4.5 ml of WB),
using a refrigerated high speed centrifuge, with speeds reaching up to 30,000 rpm (rotation per
minute). Since the lower centrifugal force (less than 300 x g) did not release a sufficient amount of
PRP, 300 × g for 5 minutes at 18°C
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