Standart Operating Protocol_Hatchery Working Flowchart.pptx

How to work in Fish Hatchery Protocol

1. Hatchery Working Flowchart A. Spawning: Land preparation, including repairing ponds, cleaning leftover fish (wild), drying, sterilization and filling with water Spreading of broodstock maximum 2.5 kg/m3, counting the number when lowering Feeding broodstock Capturing larvae begins between the 12th and 14th days, carried out by 2 people Storing larvae in fiber tanks (PN tanks) while counting the number of larvae by sampling when spreading in SR ponds B. Monosex : Making methyl testosterone solution Preparing the sex reversal pond by drying, cleaning from mud and remaining seeds, sterilizing and filling with water with a water height of approximately 1 meter Spreading larvae that have been degraded, sampled and counted Feeding the larvae with feed that has been mixed with methyl testosterone hormone for approximately 17 to 21 days Preserving for 1 day before the monosex seeds are harvested Sending seeds to PGO using a tub carried by L300 (pick up) filled with water and given salt with a salinity of 3 ppm Harvesting is done by netting with black hapa from above to reduce fish stress Conducting SR seed sampling 9. Lowering seeds into the PGO pond using a bucket, thus reducing the risk of injury