Sterilisation system complete Presentation.pptx

Sterilisation system education

1. Sterilization Any Process That Eliminates Or Kills All Forms Of Life, Including Transmissible Agents Such As Fungi, Bacteria, Viruses, Spore Forms, Etc. *SURGICAL INSTRUMENTS HAVE TO BE CLEAN FIRST BEFORE STERILIZATION*

2. METHODS OF STERILIZATION FOR SURGICAL INSTRUMENTS

3. STEAM UNDER PRESSURE STERILIZATION (AUTOCLAVE) 1. TEMPERATURE TIME PRESSURE 121°C 15-30 min 15 psig 134°C 3-4 min 28psig Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. Advantage Most frequently used and non toxic method Disadvantage Cannot be use for heat sensitive material

4. ETHYLENE OXIDE GAS STERILIZATION 2. Ethylene oxide alkylate cellular constituents of organisms, such as nucleic acid and functional proteins, including enzymes, which leads to consequent denaturation. Advantage Heat sensitive material can be sterilize. Disadvantage Carcinogenic gas is used CONCENTRATION OF GAS TIME TEMPERATURE RELATIVE HUMIDITY 450-1200mg/L 1-6hrs 37°C-63°C 40-80%

5. LIQUID CHEMICALS STERILIZATION 3. They act through alkylation of amino-, carboxyl- or hydroxyl group, and is most likely to damage nucleic acids. Advantage Less expensive method Disadvantage Monitoring the liquid sterilization process is virtually impossible CHEMICAL CONCENTRATION TIME TEMPERATURE RELATIVE HUMIDITY Formaldehyde 8% 8-12 hours 60°C-80°C 75-100% Glutaraldehyde 2%-4%

6. HYDROGEN PEROXIDE GAS PLASMA STERILIZATION (H2O2) 4. It acts on the microorganisms through its release of nascent oxygen. Hydrogen peroxide produces hydroxyl-free radical that damages proteins and DNA. Advantage Fast processing, low temperature Disadvantage Sterilant has poor penetrability TIME TEMPERATURE 28-75 min 37°C - 44°C

7. GAMMA STERILIZATION (COBALT-60) 5. High-energy radiations from Co- 60 damage the nucleic acid of the microorganism. Advantage Only parameter to control is exposure time Disadvantage Quite impractical for everyday use DOSE EXPOSURE LOW DOSE RADIATION DOSIMETER SHOW EXPOSURE OF >25 KGY HIGH DOSE RADIATION DOSIMETER SHOW EXPOSURE OF >8 TO 10 KGY

8. “Precaution is better than cure” -Edward coke It is your turn to take a step towards the safety of yourself, your team and your patients

9. Thank you for your attention Any questions?