Sterilization and disinfection

1. DIPAK KUMAR DASH
PRODUCTION OFFICER
MARCK BIOSCIENCE
GOA
11

2. DISINFECTION & STERILIZATION
 STERILIZATION
Process of
removing microbes
inclusive of
bacterial
endospores.
DISINFECTION
process of
removing microbes
except bacterial
endospores.
2

3. Physical method
Heat Radiation
Dry heat
Moist heat
Autoclave
STERILIZATION / DISINFECTION
33

4. DRY HEAT STERILISATIONDRY HEAT STERILISATION

Incineration Incineration

Over bakingOver baking
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5. MOIST HEAT STERILISATIONMOIST HEAT STERILISATION

AutoclavingAutoclaving

Boiling Boiling
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6. RADIATIONRADIATION
It is of two type that is It is of two type that is
1.1.Ionize radiationIonize radiation
2.2.Nonionizing radiationNonionizing radiation
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7. FILTRATIONFILTRATION

Membrane filter with pore size Membrane filter with pore size
between o.2 to 0.45 micrometer.between o.2 to 0.45 micrometer.

Use for heat labile liquid.Use for heat labile liquid.
Ex-serum, anti biotic sol.Ex-serum, anti biotic sol.
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8. TYPE OF FILTER ARETYPE OF FILTER ARE
1.1.EARTHENWARE FILTERSEARTHENWARE FILTERS
2.2.ASBESTOS FILTERASBESTOS FILTER
3.3.SINTERED GLASS FILTERSSINTERED GLASS FILTERS
4.4.MEMBRANE FILTERSMEMBRANE FILTERS
5.5.AIR FILTERSAIR FILTERS
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9. VIBRATIONVIBRATION

Sound waves of frequency >20,000 Sound waves of frequency >20,000
cycle/second kills bacteria and some cycle/second kills bacteria and some
viruses exposing for one hour.viruses exposing for one hour.

Use to disinfect instrument.Use to disinfect instrument.
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10. CHEMICAL STERILISATIONCHEMICAL STERILISATION

ETHYLENE OXIDEETHYLENE OXIDE

FORMAL DEHYDEFORMAL DEHYDE

BETAPROPIOLACTONEBETAPROPIOLACTONE
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11. ETHYLENE OXIDEETHYLENE OXIDE

It is an alkylating agent.It is an alkylating agent.

It act by alkylating sulphydryl,amino, It act by alkylating sulphydryl,amino,
carboxyl groups.carboxyl groups.

It is highly flammable since it is use in It is highly flammable since it is use in
mixture with carbon dioxide.mixture with carbon dioxide.
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12. USEUSE

It is to sterilize rubber and plastic It is to sterilize rubber and plastic
materialmaterial
DISADVANTAGE.DISADVANTAGE.
highly toxic,iritate eyehighly toxic,iritate eye

1212

13. FORMAL DEHYDEFORMAL DEHYDE

It use to disinfect interiors of the It use to disinfect interiors of the
room.room.

In high concentration sporicidal.In high concentration sporicidal.
1313

14. BETAPROPIOLACTONEBETAPROPIOLACTONE

It’s a sporicidal product at a conc. of It’s a sporicidal product at a conc. of
1.5 mg/kg.1.5 mg/kg.
1414

15. DisinfectantDisinfectant

Disinfectant are the substance that Disinfectant are the substance that
are applied to non living objects to are applied to non living objects to
destroy microorganism.destroy microorganism.

It is less effective than sterilization.It is less effective than sterilization.
1515

16. Commonly used Commonly used
disinfectantdisinfectant
1.1.AlcoholAlcohol
2.2.phenolphenol
3.3.ChlorhexaneChlorhexane
4.4.GlutaraldehydeGlutaraldehyde
5.5.Povidon-iodinPovidon-iodin
6.6.chloroformchloroform
1616

17. SURFACE SURFACE
DISINFECTIONDISINFECTION

Agents that damage bacterial cell Agents that damage bacterial cell
membranes may be used to disinfect membranes may be used to disinfect
inert surfaces or skin.inert surfaces or skin.

Ex Ex
detergents,alcoholdetergents,alcohol
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18. CONCLUSIONCONCLUSION

All sterilization processes must be All sterilization processes must be
validated.validated.

These process should be care fully These process should be care fully
done for effective product.done for effective product.
1818

19. REFERENCESREFERENCES

Sharma P.P; How to practice GMPs; (Good Manufacturing Practices); Fourth Sharma P.P; How to practice GMPs; (Good Manufacturing Practices); Fourth
Edition ; Vandana Publication pvt. Ltd. Delhi. pp-208-280Edition ; Vandana Publication pvt. Ltd. Delhi. pp-208-280

Potdar M.A ; cGMP; (Current Good Manufacturing Practices) For Potdar M.A ; cGMP; (Current Good Manufacturing Practices) For
Pharmaceuticals; Pharma Med Press; Hyderabad-500095. pp-339-384Pharmaceuticals; Pharma Med Press; Hyderabad-500095. pp-339-384

Shah D.H.; SOP Guidelines; straight and simple; 1Shah D.H.; SOP Guidelines; straight and simple; 1
stst
edition; published by edition; published by
Business Horizons;G-59, New Delhi-110048, pp-161-183.Business Horizons;G-59, New Delhi-110048, pp-161-183.

Mallik V; Drugs& Cosmetics Act 1940; 18Mallik V; Drugs& Cosmetics Act 1940; 18
thth
edition;2005; published by edition;2005; published by
Eastern Book Company; Lucknow.Eastern Book Company; Lucknow.
1919

20. 2020