Sterilization: The removal of all microorganisms and other pathogens from an object or surface by treating it with chemicals or subjecting it to high heat or radiation. Sterilization also refers to procedures that result in infertility. Types of sterilization: 1.Dry Heat 2.Steam sterilizer 3.Chemical sterilizer 4.Glass bead sterilizer 5.Laboratory sterilizer 2
Methods of Sterilization: The various methods of sterilization are: 1.Physical Method: a . Thermal Method b . Radiation method c . Filtration method 2.Chemical Method: a . Gaseous method. 3
1.Dry heat sterilization : - It was the first method of sterilization and is a longer process than moist heat sterilization. The destruction of microorganisms through the use of dry heat is a gradual phenomenon. 2.Steam Sterilization : The steam Sterilization depend on the hot air/steam. Kill the microbs using steam. 3.Chemical Heated Sterilization: The elimination of viable Microorganism an their spores using chemical liquids and gaseous compounds. 4
Glass Bead Sterilization: In the glass bed sterilization the sterilization done by using glass container which contain glass bed and heated it . about 437F. Laboretory Sterilizer: The sterilization is done by using the lab . apparatus. e.g . Autoclave, Ovens, UV Chamber. 6. 5
Advantages: 1.Prevent Transmission of Diseases. 2.Prevant Contamination. 3.Prevant growth of undesirable Bacteria. 4.Prevant spoilage of material by microbs . 6
DISADVANTAGES 1.It is high in a cost. 2.High chances of accidents. 3.At that time operation chances of causes the Respiratory disease. 4.Chances of Skin Burning. 5.Showing problem to handling.
Application: 1.Foods -One of first step to words the sterilization was made by Nicols Apart who discover that through application of heat over suitable period. 2.Medicine and Surgery: -It is used to preparation of injectable medication. 3.Laboratory: -It used in labs. To cleaning apparatus or glassware's. 8. 7