The Basics of Biosafety and Bioethics.pptx

the general rules to be followed inside the biotechnology lab

1. DEPARTMENT OF BIOTECHNOLOGY MARY MATHA COLLEGE OF ARTS AND SCIENCE, PERIYAKULAM By Dr. S. HIMALINI

2. BIOSAFETY AND BIOETHICS

3. Safety Rules NO food or drink in the lab. NO horseplay or loud voices in the lab. Keep workstation neat and clean. Understand what you are doing in the lab before beginning the lab experiment. Keep hands away from face while working. Know emergency procedures in case of an emergency. Work with a partner, but produce your OWN work. Wash your hands before leaving the lab.

4. General Guidelines for Lab Safety Only perform experiments specifically assigned by your teacher . Understand safety rules before entering the lab. Tie back hair, roll up sleeves, put on and leave on, any required safety protective equipment for each lab.

5. Know the location of all safety and emergency equipment used in the laboratory. Immediately report and accident, incident, or hazard – no matter how trivial –to teacher . In case of fire, alert your teacher and leave the lab following given procedure. Do not eat or drink in the lab .  

6. Chemical Safety Always wear safety goggles, gloves, and a lab apron or coat when working with any chemicals. Do not touch, taste, or smell any chemicals unless instructed by your teacher. Know location of eye wash and shower stations. Do not mix any chemicals unless instructed to do so by your teacher.

7. Do not pour water into a strong acid or base. Pour acid into the water. Use of Chemicalsand Biological Agents   Know the properties of chemicals and biological agents you use before you use or transport them • Toxicity • Flammability • Reactivity/Incompatibilities • Corrosive • Unstable • Radioactive • Clean up procedures

8. Eye Safety   WEAR SAFETY GOGGLES as directed. • Do not touch your eyes or face during lab and always wash your hands after performing a lab Safety with Gases or Inhalants   Should use a fume hood! Do not inhale any gas or vapor Handle materials that emit vapors or gases in a well ventilated area . Heating Safety   Hot plates look the same hot or cool. NEVER touch a hot plate to determine its temperature. ALWAYS assume hotplates are hot and treat them as such.

9. Sharp-Object Safety   Use extreme care when handling all sharp and pointed instruments, such as scalpels, sharp probes, and knives. Do not cut objects while holding them in your hand. Cut objects on a suitable work surface. Always cut in a direction away from your body .

10. Hygienic Care   Keep hands away from your face and mouth while working in the lab. Wash your hands before leaving the lab. Use proper technique when handling bacteria or other microorganisms. These are pathogens!!! Keep petri dishes sealed. Dispose of petri dishes as instructed by teacher for proper disposal. Wash hands after touching any cultures dish! Spill of caustic material on clothing  

11. Glassware Safety   Inspect glassware for cracks BEFORE using. Notify teacher immediately if glassware breaks Do not attempt to pick it up with hands. Dispose of broken glassware in specified container. This container.

12. Clean up and Proper Waste Disposal   Clean and Sanitize all work surfaces and safety attire after each lab period. Dispose of all sharp objects and contaminated materials as directed by your teacher .

13. Good lab practices

14. SAFETY MEASURES

15. THANK YOU