The Clean Living Project Episode 20 - Methylene Blue

From an industrial fabric dye, to the first synthetic drug ever used for medicinal purposes. Methylene Blue was first known as a highly effective anti-parasitic and a treatment for Malaria. It’s now being studied for its effects on depression, cancer, cognitive function and enhancement, dementia, Alzheimer’s, Autism, heart disease, viruses such as COVID, herpes and HIV and much more!

This smurfy blue substance is all the rage these days, so come find out what it’s all about!

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WHAT IS METHYLENE BLUE?

Methylene blue is a synthetic compound with a deep blue color,
commonly used as a dye in laboratories and medicine. | was first 1
, synthesized in 1876 as a chemical dye for the textile industry.
In 1891, scientist Paul Ehrlich discovered it was anti-parasitic,
. leading MB to become a treatment for Malaria, making it the Ist
synthetic drug ever used for medicinal purposes.

Today, MB has a variety of applications, including staining
biological specimens for microscopic examination and as a redox
indicator in chemical reactions and is extensively studied for its
diverse therapeutic properties. It’s used as an antidote for cyanide
poisoning, a urinary antiseptic, and more recently, it’s gained
interest for its neuroprotective and cognitive-enhancing effects and.

implications as a highly effective metabolic medicine. CLE

3. MITOCHONDRIA & ATP

Dubbed the “powerhouse of the cell,” mitochondria produce the primary
energy currency of cells - adenosine triphosphate (ATP). 90% of the
energy you produce comes from Mitochondria.

Dysfunction in mitochondria can lead to various diseases and conditions,

including:

+ Mitochondrial Diseases

. Neurodegenerative Diseases: such as Parkinson's disease,
Alzheimer's disease, and amyotrophic lateral sclerosis (ALS)

« Metabolic Disorders: such as diabetes, obesity, and metabolic
syndrome

. eue Diseases: such as heart failure and ischemic heart

isease

+» Aging: Accumulation of mitochondrial DNA damage and decline in

mitochondrial function are associated with the aging process and

age-related diseases. a

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Electron Transport Chain (ETC)

Mitochondria produce ATC in the ETC, consider it the power station
where energy is generated.

y To power the ETC, our body goes through a process called Cellular
a Respiration. It starts with breaking down the food we eat into

: ‘= molecules like Pyruvate and Acetyl CoA. These molecules then enter

the Krebs Cycle, where they're converted into NADH and FADH2—

these are like the fuel for the ETC.

The ETC consists of enzymes on the inner membrane of the

mitochondria. They use the energy from NADH and FADH2 to pump

protons out of the membrane. Then, the protons flow back in through
another enzyme called ATP Synthase, generating ATP—our body's

energy currency. 1
But sometimes, a small percentage of electrons "slip" out of the ETC, e
creating reactive oxygen species (ROS). These are like small sparks — '.:
—some are good for us, but too many can damage cells and DNA.

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METHYLENE BLUE & MITOCHONDRIA

Methylene blue aids mitochondria by accepting electrons in the
electron transport chain (ETC), enhancing the flow of electrons and
ultimately promoting ATP production, which is essential for cellular
energy metabolism. This action can help improve mitochondrial
function, particularly in conditions where mitochondrial dysfunction
is present. Furthermore, methylene blue's antioxidant properties
enable it to scavenge harmful reactive oxygen species (ROS)
generated during mitochondrial respiration, thereby protecting
mitochondria from oxidative damage and maintaining their
integrity. By supporting mitochondrial function and reducing
oxidative stress, methylene blue may contribute to cellular health
and potentially alleviate symptoms associated with mitochondrial
dysfunction-related diseases.

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METHYLENE BLUE + LIGHT THERAPY

Methylene blue can enhance the effects of red light therapy
by acting as a photosensitizer. When methylene blue is
exposed to red light, it undergoes a process called
photodynamic therapy, where it absorbs the light energy and
produces positive reactive oxygen species (ROS). These
types of ROS can have therapeutic effects, such as
promoting wound healing, reducing inflammation, and
potentially killing cancer cells. Therefore, combining
methylene blue with red light therapy can enhance the
therapeutic benefits of both treatments, particularly in
conditions where ROS-mediated effects are desired.

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7. TA SN
CANCER AS A METABOLIC DISEASE

“Cancer: The Metabolic Disease Unraveled” is a book that
explores the theory that cancer is primarily a metabolic disease
rather than a genetic one. The author, Thomas Seyfried, argues
that cancer is caused by damage to the mitochondria, the
energy-producing structures in cells, leading to dysfunctional
cellular metabolism. This metabolic dysfunction causes cells to
switch from their normal, oxygen-based metabolism to a more
primitive, glucose-based metabolism, which allows them to grow
and spread uncontrollably.

Studies have shown that Mitochondria transferred from cancer
cells into normal cells turned them into cancer cells, and
mitochondria transferred from normal cells into cancer cells
reverted cancer cells back to normal cells. 21

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METHYLENE BLUE AND CANCER PF?!

+ Antiproliferative Effects: Methylene blue has been shown to inhibit the
proliferation of cancer cells in various types of cancer, including breast
cancer, prostate cancer, and glioblastoma.

| + Induction of Apoptosis: Methylene blue can trigger programmed cell death
(apoptosis) in cancer cells, leading to their destruction.

+ Inhibition of Angiogenesis: Methylene blue has been found to inhibit the
formation of new blood vessels (angiogenesis) that are essential for tumor
growth and metastasis, thereby impeding cancer progression.

+ Disruption of Mitochondrial Function: Methylene blue can interfere with
mitochondrial function in cancer cells, disrupting their energy production and
inducing cytotoxic effects.

+ ROS Generation: Methylene blue can generate reactive oxygen species

oxidative damage to cancer cells, contributing to their destruction. ¿>
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(ROS) when activated by light in photodynamic therapy. ROS can cause

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METHYLENE BLUE AND CANCER P"! 2

+ Synergistic Effects with Other Treatments: Methylene blue has been
shown to enhance the effectiveness of other anticancer therapies, such as
chemotherapy and radiotherapy, when used in combination.

+ Selective Toxicity: Methylene blue exhibits selective toxicity towards cancer

cells while sparing normal cells. This property is advantageous in cancer

treatment as it minimizes adverse effects on healthy tissues.

Inhibition of Tumor Growth: Animal studies and some clinical trials have

ny demonstrated that methylene blue can inhibit tumor growth and metastasis

in various cancer models.

Inhibition of Cancer Stem Cells: Methylene blue has been reported to

target cancer stem cells, a small population of cells within tumors believed to

drive tumor growth, recurrence, and resistance to therapy.

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METHYLENE BLUE & METHEMOGLOBINEMIA

Methemoglobinemia is a condition characterized by elevated levels of
methemoglobin, a form of hemoglobin that cannot bind oxygen
21 cffectively. This leads to impaired oxygen delivery to tissues, resulting
in symptoms such as cyanosis (bluish discoloration of the skin),
Y shortness of breath, dizziness, and in severe cases, organ failure.

Methylene blue acts as a reducing agent, converting methemoglobin
back to its normal form, hemoglobin, which can effectively bind and
transport oxygen. When administered intravenously, methylene blue
quickly reduces methemoglobin levels, restoring oxygen-carrying
capacity and alleviating symptoms of methemoglobinemia. This
treatment is particularly effective for acquired methemoglobinemia
caused by exposure to certain drugs, chemicals, or toxins.

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11. En. e.

MB TREATMENT FOR NEURODEGENERATIVE DISEASES

¢ Antioxidant Properties: Methylene blue acts as an antioxidant,
scavenging free radicals and reducing oxidative stress, which is
implicated in the progression of neurodegenerative diseases such as
Alzheimer's and Parkinson's.

of neurodegenerative diseases.
Tau Protein Modulation: Methylene blue interferes with the
aggregation of tau protein, which forms neurofibrillary tangles in
pr; dira disease. By preventing tau aggregation, methylene blue may
slow disease progression

+ Neuroprotective Effects: Methylene blue has been shown to protect
neurons from damage and death in various experimental models of
neurodegeneration, potentially through mechanisms involving anti-
inflammatory and anti-apoptotic actions.

+ Enhancement of Neurogenesis: Methylene blue may promote
neurogenesis, the generation of new neurons, in the brain. CLE

mu Mitochondrial Function: Mitochondrial impairment is a common feature
| .
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MB ON FIGHTING INFECTIONS

¢ Antibacterial Activity: Methylene blue PDT has been effective against
a wide range of bacteria, including both Gram-positive and Gram-
negative species. It can penetrate bacterial cells and disrupt their
membrane integrity, leading to cell death.

+ Antifungal Activity: Methylene blue PDT has also shown efficacy
against various fungal pathogens, including Candida species. It disrupts
fungal cell membranes and inhibits fungal growth.

+ Antiviral Activity: Methylene blue PDT has demonstrated antiviral
activity against certain viruses. It can inactivate viruses directly or
indirectly by damaging viral envelopes or nucleic acids.

+ Biofilm Disruption: Methylene blue PDT can disrupt microbial biofilms,
which are communities of microorganisms encased in a protective Li
matrix. This is particularly important as biofilms contribute to the
persistence of intections and antibiotic resistance.

13. ENHANCING COGNITIVE FUNCTION AND MOOD

Methylene blue may enhance cognitive function and improve
mood through its actions as a neurotransmitter modulator and
antioxidant. It can influence neurotransmitter systems in the
brain, such as serotonin and dopamine, which are involved in
mood regulation and cognitive processes. Additionally, methylene
blue's antioxidant properties help protect brain cells from
oxidative damage, which can contribute to improved cognitive
function and mood stability.

Chronic inflammation has been implicated in various neurological
disorders and mood disturbances. Methylene blue exhibits anti-
inflammatory properties, modulating immune responses and
reducing inflammatory signaling pathways. By mitigating
neuroinflammation, methylene blue may contribute to
neuroprotection, improved neural plasticity, and mood

regulation.

14. A

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METHYLENE BLUE AND AUTISM

Several lines of evidence suggest that oxidative stress and
mitochondrial dysfunction may contribute to the pathophysiology
of autism spectrum disorder (asp) Methylene blue's antioxidant
properties make it a potential candidate for mitigating oxidative
+ and protecting against neuronal damage associated with
ASD.

Preclinical studies in animal models of ASD have shown promising
results with methylene blue administration, includin
improvements in behavioral symptoms, cognitive function, pi
social interactions. These effects may be attributed to methylene
blue's ability to reduce oxidative stress, enhance mitochondrial
function, and modulate neurotransmitter systems in the brain.
Additionally, a few small clinical trials and case reports have
explored the use of methylene blue as a therapeutic intervention
for individuals with ASD reporting positive outcomes in terms of
behavioral improvements and cognitive functioning.

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15. eE) METHYLENE BLUE AND VIRUSES

Methylene blue fights viruses by directly damaging viral
structures, generating reactive oxygen species (ROS) that
can inactivate viruses by damaging their envelopes or
nucleic acids, and interfering with viral replication
processes within infected cells, inhibiting the production of

new virus particles.
N Methylene blue has been known to inactivate viruses such
pe as herpes, West Nile, Zica, Ebola, Hepatitis C and A, HIV
E and Covidl9.

16. METHYLENE BLUE ANTI-ANGING PROPERTIES

Methylene blue acts as a nitric oxide inhibitor, regulating
its levels and reducing oxidative stress associated with
aging. Additionally, it functions as an estrogen antagonist,
meaning it competes with estrogen for binding sites on
estrogen receptors, thereby blocking estrogen's effects.
MB inhibits estrogen signaling pathways involved in aging-
related processes such as inflammation. and cell
proliferation. By modulating these pathways, methylene
blue may help impede the aging process and promote
cellular health.

|

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17. METHYLENE BLUE EFFECTS ON HORMONES

+ Inhibits prolactin: Inhibiting prolactin production can help
control tumor growth, alleviate irregular menstrual periods,
infertility, PCOS or certain psychiatric disorders.

Inhibits estrogen: helps manage conditions such as

estrogen receptor-positive breast cancer endometriosis or
uterine fibroids

Increases thyroid hormones: treats hypothyroidism and
restores metabolic function and energy levels

Increases testosterone: helps address symptoms of
hypogonadism, such as low libido, fatigue, and reduced
muscle mass u

18. NITRIC OXIDE

Nitric Oxide is a free radical found both in the body
and in environmental toxins.

+ Nitric Oxide is central to cancer. One way to turn a
healthy cell into a cancer cell is to expose it to the
environmental pollutant nitric oxide.

+ Signs point to Nitric Oxide being the primary cause of
aging in every cell and tissue of the body.

+ Methylene Blue is a nitric oxide inhibitor and powerful
antioxidant and has proven itself useful for remedying
mitochondrial disfunction perhaps better than any
other known medicine.

20. DOSING

Pharmaceutical Grade ONLY
.5mg to 2mg per kg in divided doses
Do not take if you take SSRI drugs

Consult your DR

21. STUDY: THE EFFECT OF METHYLENE BLUE PHOTODYNAMIC THERAPY
TREATING CANCEROUS AND NON-CANCEROUS SITUATIONS

In the study, methylene blue photodynamic therapy (MB-PDT) was found to be effective in
treating both cancerous and non-cancerous situations. The therapy involves the use of a
photosensitizer, in this case, methylene blue, which is activated by light of a specific
wavelength. This activation leads to the production of reactive oxygen species that can
cause cell death in the targeted area.

In cancerous situations, MB-PDT was found to selectively target and kill cancer cells while
sparing the surrounding healthy cells. This selectivity is due to the higher uptake of
methylene blue by cancer cells compared to normal cells. The therapy was effective in
treating various types of cancer, including breast cancer, and could potentially be used as
an adjunct therapy to surgery to increase the eradication rate of microscopic residual
disease.

In non-cancerous situations, MB-PDT was found to be effective in treating various
conditions, such as oral mucositis related to cancer treatment and skin cancer. In these
cases, the therapy was able to reduce pain and promote healing by selectively targeting
and killing the affected cells.

Overall, MB-PDT was found to be a promising treatment option for both cancerous and non-
cancerous situations due to its selectivity, ability to target specific cells, and its potential
to cause minimal side effects.

22. STUDY: THE EFFECT OF METHYLENE BLUE AND BLACK SEED OIL IN TREATING
BACTERIAL AND CORONAVIRUS INFECTIONS IN-VIVO AND IN-VITRO

In the study, researchers found that a combination of methylene
blue and black seed oil can effectively treat bacterial and
coronavirus infections in both laboratory settings (in vitro) and in
living organisms (in vivo).

The study showed that the combination of these two substances
was able to kill various types of bacteria and also inhibit the
growth of coronaviruses, including the one that causes COVID-19.
The combination was more effective than using either substance
alone. In mice, the treatment was able to reduce the number of
bacteria in the lungs and improve the survival rate of the animals.

23. STUDY: THE EFFECT OF METHYLENE BLUE ON SEPSIS

In the study, methylene blue was found to be effective in
treating sepsis, a serious condition caused by the body's
response to an infection. Methylene blue works by reducing
inflammation and improving blood flow in the body. It also
helps to improve the function of mitochondria, which are the
energy-producing parts of cells. In the study, methylene blue
was shown to improve the survival rate of animals with
sepsis and to reduce the amount of damage to their organs.
Overall, the findings suggest that methylene blue could be a

promising treatment option for sepsis in humans.
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24. STUDY: METHYLENE BLUE ENHANCES RESTING-STATE
FUNCTIONALITY OF THE BRAIN'S NEURAL CONNECTIONS
Researchers found that methylene blue can improve the communication
between different parts of the brain during rest. They used a technique
called resting-state functional connectivity magnetic resonance imaging
(rs-feMRI) to measure the brain's activity while participants were resting.

The study showed that when participants took methylene blue, the
communication between different parts of the brain was better than
when they took a placebo. The researchers believe that this
improvement could be due to methylene blue's ability to increase the
production of a chemical called adenosine triphosphate (ATP), which is
important for brain function.

Overall, the findings suggest that methylene blue could be a useful
treatment for conditions that affect the brain's communication, such
depression and dementia. CLE

25. NERO 2

STUDY: THERAPEUTIC BENEFITS OF METHYLENE BLUE
ON COGNITIVE IMPAIRMENT DURING CHRONIC
CEREBRAL HYPOPERFUSION

This study found that methylene blue can improve blood flow
in the brain and may help protect against Alzheimer's disease.
Cerebral hypoperfusion, or reduced blood flow to the brain, is
a risk factor for Alzheimer's. The study showed that methylene
blue can increase blood flow and oxygen delivery to the brain,
which may help prevent the damage associated with
Alzheimer's disease. Overall, the findings suggest that
methylene blue could be a potential treatment for preventing
or slowing the progression of Alzheimer's disease.

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26. STUDY: PROTECTION AGAINST NEURODEGENERATION WITH
LOW-DOSE METHYLENE BLUE AND NEAR-INFRARED LIGHT

This «study shows that methylene blue can help treat
depression by improving the function of mitochondria, which
are the energy-producing parts of cells. The study involved 15
patients with depression who were given methylene blue for
two weeks. The patients showed improvements in their
depression symptoms, and the researchers believe that this
improvement is due to methylene blue's ability to improve
mitochondrial function. Overall, the findings suggest that
methylene blue could be a promising treatment for depression.

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STUDY: THERAPEUTIC BENEFITS OF METHYLENE BLUE
ON LIVER CIRRHOSIS

In the study, researchers found that methylene blue can help
treat liver cirrhosis, a condition where the liver becomes
scarred and doesn't function properly. Methylene blue works
by reducing inflammation and improving blood flow in the
liver. The study showed that methylene blue treatment
improved liver function and reduced the amount of scarring in
the liver in animal models of liver cirrhosis. Overall, the
findings suggest that methylene blue could be a potential
treatment for liver cirrhosis in humans.

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28. STUDY: THERAPEUTIC BENEFITS OF METHYLENE BLUE
ON SKIN HEALTH AND LONGEVITY

In the study, researchers found that methylene blue has
significant potential for improving skin health and longevity.
Methylene blue was shown to promote skin cell proliferation,
increase skin hydration, and stimulate the production of
collagen and elastin, which are essential for maintaining
youthful and healthy skin. It also protects the skin against UV
damage and has shown promising results in reversing aging in
skin cells from older individuals. Overall, the findings suggest
that methylene blue could be a promising compound for
promoting skin longevity and improving skin health.

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29. STUDY: THERAPEUTIC BENEFITS OF METHYLENE BLUE
FOR FIGHTING DEPRESSION

This study shows that methylene blue can help treat
depression by improving the function of mitochondria, which
are the energy-producing parts of cells. The study involved 15
patients with depression who were given methylene blue for
two weeks. The patients showed improvements /in their
J depression symptoms, and the researchers believe that this
Y improvement is due to methylene blue's ability to improve
mitochondrial function. Overall, the findings suggest that
—- methylene blue could be a promising treatment for depression.

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