The Procedure and Classification of Column Chromatography Process

In terms of the nature of the solvent flow in the column, the process is categorized in two different types. When the flow of the solvent in the column is managed by gravity or by percolation, the process is called gravity column chromatography.

1. The Procedure and
Classification of Column
Chromatography Process

2. •Inthechemicalindustry,theprocessofpurificationplays
averycrucialrole.Theprocessmainlyrefersto
separatingtwodifferentcomponentsforchemicaluse.
Separatingtwodifferentcomponentswhicharenot
structurallysimilarisnotabigdeal.

3. Adsorbents for
Chromatography
Silica Gel
Chromatography
Thin Layer
Chromatography
Aluminium Oxide
Chromatography
Column
Chromatography

4. •Butincaseswherethestructureandnatureofthe
componentsaresimilar,thepurificationprocess
needssomeadvancedtechnology.Column
chromatographyisatechniquethatisusedto
separatetwoalmostsimilarnaturecomponents.

5. •Oneofthekey
advantagesofthis
processisthatitis
extremelycosteffective.
Thecolumn,usedinthis
processismadeofglass
withadiameterof5mm
to50mm.Andthe
heightofthecolumnis
somewherebetween5
cmto1meter.Thereisa
tapinthebottomas
well.

6. •Basicallytwomethods
arefollowedtoprepare
thecolumnandthose
aredrymethodandwet
method.Inthisprocess
thestationaryphaseisa
solidadsorbentmostly
silicageloraluminium.
Itisplacedinthe
columnvertically.Then
thereisthemobile
phasewhichisnormally
liquid.