Transformasi bakteri biologi sel mol.pptx

Transformasi bakteri

1. Transformasi bakteri Probiselmol 2012

2. In nature, genes can be transferred between bacteria in three ways: Conjugation – mating process during which genetic material is transferred from one bacterium to another of a different mating type Transduction – a virus acts as a vector (carrier) to transfer small pieces of DNA from one bacterium to another Bacterial Transformation – involves the transfer of genetic information into a cell by direct uptake of the DNA (occurs only rarely in nature)

3. Transformation in the Laboratory Transformation was first performed in the laboratory by Griffith and later by Avery,

4. Bacterial growth curve

5. Bacteria can take up DNA only during the period a the end of logarithmic growth – cells are said to be competent (can accept DNA that is introduced from another source)

6. Bacteria cell membrane is weakened using ice cold CaCL 2 cells are then “heat shocked” to induce them to take up the plasmid