•Student will be able to,
•Define translation.
•Discriminate transcription and
translation.
•Explain the process of
translation.
•Draw initiation complex.
•Transcription- It is the process of
formation of RNA from DNA
•Diagram:
DNA
RNA
PROTEIN
REVERSE
TRANSCRIPTION
TRANSCRIPTION
TRANSLATION
•What is translation ?
•Key players of translation
•Steps in translation
•Charging (activation of A.A)
•Initiation
•Elongation
•Termination
•It is the process by which genetic
information coded in m-RNA
converted in the form of a
polypeptide.
•Ribosome
•M-RNA
•T-RNA
•Amino acid
•These are
ribonucleoprotein
particle, provide
the site for protein
synthesis.
•Messanger RNA carry the information
in the form of genetic code for a
specific protein required by body.s
•Structure:
•Transfer RNA
•Structure:Cloverleaf model of t-RNA
•There are 20 amino acid
identified in our body.These are
the unit of protein, aggregate by
the process of translation to form
a polypeptide.
C
NH2
H
RCOOH
•Translation of m-RNA begins with
the formation of initiation complex.
•It involves :Smaller sub unit of
ribosome, m-RNA,Charged t-
RNA,GTP,Mg2+,Initiation factors Ifs.
I
•Diagrams
•Diagram:
NH
2
C
H
R COOH CHNH R
H
COOH
NH
2
C
H
R C R
H
COOH
C
O
N
H
+ H
2O
Polypeptide chain is terminated
when t-RNA find termination codon
on m-RNA.
Termination codons are
AAU, AGU, AUU
•In translation process many ribosomes
are attached in m-RNA and formed
polysome.
•Structure:
•Define Polysome.
•What is peptide bond ? How it
formed ?
•Name the structures involved in
the formation of initiation
complex
•What are initiation and
termination codon ?