TRICHOMONIASIS.pptx/TRICHOMONIASIS.pptx h

TRICHOMONIASIS.pptx

1. TRICHOMONIASIS

2. Trichomonads are flagellate protozoa with 3-5 anterior flagella Flagellates exists only in the trophozoite phase and there is no cystic phase Classified in to 3 species according to their habitats

3. Trichomonas vaginalis (13µm)- inhabiting the female genital tract and urinary tract of males and females Trichomonas tenax- inhabiting the oral cavity Trichomonas hominis- inhabiting the ileocaecal region

4. Trichomonas vaginalis MORPHOLOGY: As the trophozoite and pear shaped and measure 10-30µm in length and 5-20µm in breadth Anterior end is round and cleft like depression is present inside Single ovoid nucleus is present at the anterior end 4 anterior flagella are free posterior end is pointed Thicker flagellum passes backward along the side of the body and forms undulating membrane which reaches up to the middle of the body

5. Undulating membrane is supported at the base by rod like structure named “Costa’’ Axostyle runs down the middle of the body and ends in the pointed tail like extremity Motile by jerky movement Divides by binary fission Trophozoite is infective form as there is no cystic form

6. Pathogenesis Trichomoniasis in females In male urethritis Often asymptomatic in males In female produce vaginitis with offensive yellowish discharge parasite transmitted sexually

7. Laboratory diagnosis MICROSCOPIC EXAMINATION: Vaginal or urethral discharge – examined with drop of saline for characteristic motile trophozoites with jerky movements In males may found in urine or prostatic secretions Fixed smears stained with P apaniculou , L eishman and Giemsa stain under light microscope Direct immunofluorescent antibody staining

8. CULTURE: Can be isolated from urethral and vaginal discharge on a variety of media CPLM (cyteine, peptone, liver, maltose) media MOLECULAR METHODS: Nucleic acid hybridization method and polymerase chain reaction

9. Prevention Detection and treatment of cases Avoidance of unprotected sexual intercourse No vaccinee

10. Treatment Metronidazole is the drug of choice

11. Thank you