Trypanosoma brucei rhodesiense

1. Trypanosoma brucei rhodesiense Prepared by: NOE P. MENDEZ CENTRAL MINDANAO UNIVERSITY (CMU) [email protected]

2. Kingdom: Protista Subkingdom: Protozoa Phylum: Sarcomastigophora Subphylum: Mastigophora Class: Zoomastigophora Order: Kinetoplatida Family: Trypanomatidae Genus: Trypanosoma Species: T. brucei Subspecies: T. b. rhodesiense TAXONOMY

3. 2 Interchangeable Form: Trypanomastigote Epimastigote MORPHOLOGY Form: Amastigotes Promastigote Epimastigote Trypanomastigote Metacyclic Trypanosome

4. Basic Form Kinetoplast (K) Axoneme (A) Nucleus (N) Volution Granules (VG) Flagellum (F) K A N Flagellar pocket (FP) Amastigote Promastigote

5. A K N Posterior End Undulating membrane (UM) F A K N F UM Epimastigote Trypanomastigote & Metecyclic Mastigote Basic Form

6. Life Cycle

7. East African Trypanosomiasis (Sleeping Sickness) Glossina morsitans (Tsetse fly) Trypanosomiasis

8. Pathogenesis and Clinical Picture Trypanosoma Chancre Via: Lymphatic Winterbottom’s Sign Blood Stream Headache, fever, muscle and joint pain, irregular erythematous rash, and hypoplastic anaemia Invasion of CNS Sever headache, mental apathy, slow speech, deep sleep, coma and death Trypanosomiasis

9. Progressive disease lead to C.N.S. manifestations: Insomnia Mood Changes (Dullness/Apathy) Motor and Sensory Disorders ( Hypersthesia /Slurred Speech/Abnormal Gait) Convulsion Epilepsy Terminal Stage: Permanent Sleep Secondary Bacterial Infection Coma & death Trypanosomiasis

10. Clinical Picture Diagnosis

11. Trypanosoma Chancre Winterbottom Sign Epilpsy Convulsion Clinical Picture

12. Clinical Picture Demonstration of Trypanosomes Microscopic E xamination Culture on S uitable Medium Animal Inoculation Diagnosis

13. Microscopic Examination Animal Inoculation C.S.F. Demonstration of Trypanosome Demonstration of Trypanosome

14. Clinical Picture Demonstration of Trypanosomes Microscopic E xamination Culture on S uitable Medium Animal Inoculation Serological Test Diagnosis

15. Serological Test

16. Early Stage Pentaminidine or Suramin Late Stage Tryparsamide Both Early and Late Stage Eflornithine (DMFO) Ornidyl Treatment

17. Treatment of Patient Control of Vectors Pentamidine as prophylactic drug Control

18. End of Report Questions?