Types of paper chromatography

Types of paper chromatography
Applications of Paper Chromatography

1. PAPER CHROMATOGRAPHY Mr. MAHADEVAPPA KAMMAR

2. CONTENTS Introduction. Types of paper chromatography . 1. Ascending chromatography. 2. Descending chromatography. 3. Ascending-descending chromatography. 4. Radial chromatography. 5. Two dimensional chromatography. Applications of Paper chromatography. Conclusion. References .

3. INTRODUCTION Dr.M.Tswett introduced paper chromatography in 1906. Paper chromatography is simple and most widely used separation technique. Partition chromatography principle . The separation is effected by differential migration of the mixture of substances, which takes place due to difference in partition. Analysis of unknown substances.

4. TYPES OF PAPER CHROMATOGRAPHY 1. Ascending Chromatography. 2. Descending Chromatography. 3. Ascending-Descending Chromatography. 4. Radial or Circular Chromatography. 5.Two dimensional Chromatography.

5. 1.Ascending Chromatography

6. 2.Descending Chromatography

7. 3.Ascending-Descending Chromatography

8. 4.Radial or Circular Chromatography

9. 5.Two dimensional Chromatography

10. APPLICATIONS OF PAPER CROMATOGRAPHY A qualitative method for identifying components of mixture. Identification of drug and impurities In forensic studies( DNA and RNA sequencing). As an analytical chemistry technique (Colored mixtures like pigments). Unknown organic and inorganic compounds. Identification of biomolecules . It’s an inexpensive and rapid method for separation.

11. CONCLUSION From this presentation, I came to know about importance and types of paper chromatography. How it is useful in separation and analysis of mixture of components. Paper chromatography is widely used in areas pharmaceutical and many chemical industries. As it is inexpensive and simple, it is basic chromatographic technique performed in laboratories.

12. REFERENCES Gurdeep R Chatwal and Sham K Anand.2013.”Instrumental methods of chemical analysis”,1 st Edition , Himalaya Publishing House. :2.590-2.592 R.P. BUDHIRAJA. Copy right 2OO4”Separation Chemistry”, New Age International (P) Ltd. 240-246 http://namrataheda.blogspot.in/search/label/types%20of%20paper%20chromatography . www.tutorvista.com/biology/uses-of-paper-chromatogarphy?view=simple