Use of Cisgenesis for vegetable crop improvement by Mukhi Vinita.pptx

Use of Cisgenesis for vegetable crop improvement

1. COURSE TITLE: PRINCIPLES OF VEGETABLE BREEDING COURSE CODE:HVSC 0504 Use of Cisgenesis for vegetable crop improvement SUBMITTED TO: Dr. Manisha Assistant Professor Department of Horticulture SUBMITTED BY: Mukhi Vinitha (230805200002) M. Sc. Horticulture (Vegetable Science)

2. Introduction The term was first introduced in 2000 by Henk J. Schouten and Henk Jochemsen . This concept was given by Dutch researchers Schouter , Krens and Jacobsen in 2006. According to this concept ,genetic material transferred to the plant should originate from the plant itself or from closely related species capable of sexual hybridization.

3. Cisgenesis Cisgenesis is a type of genetic modification wherein both donor and recipient are from the same species (crossable and sexually compatible) and a gene is transferred. Such a gene includes its introns and a flanked by its native promoter and terminator in the normal-sense orientation. Cisgenesis plants can harbor one or more cisgene but they do not contain an transgene.

4. 1.Potatoes resistant to late blight. 2.Cisgenic apple which confer scab resistance ( V arblaere et al ., 2011)   3.Cisgenic barley with improved phytase activity(Holme et al ., 2012) Examples

5. Transgene is a gene which is transformed from unrelated organism. Transgenesis is the genetic modification of a recipient plant with one or more genes from any non-plant organism, or from a donor plant that is sexually incompatible with the recipient plant. Transgenesis

6. Intragenesis In Intragenesis , different plant genetic components are recombined in vitro to produce an expression gene construct that is introduced into a plant within the same sexually compatible gene pool ( Rommens et al ., 2007).

7. Difference between conventional breeding ,transgenesis and cisgenesis

8. (Ahmad et al ., 2020)

9. Why Cisgenesis ? Natural genes from the crop species itself . Gain of time Cisgenesis may be safer than traditional breeding since the introduction of unwanted traits via linkage drag can be prevented. Genetic material of the original cultivar is preserved. Only one or few genes are added. High quality cultivar is maintained. Preferred by consumers compared to transgenesis. Specifically important for outbreeding and vegetatively propagated crops. ( Haverkort et al ., 2008)

10. Development of Cisgenic plant

11. CASE STUDY

12. Objectives : Improves the existing varities with disease resistance. Stacking of multiple R genes Materials and methods : Plant materials Potato varieties-Atlantic , Bintje ,Potae9 Five Phytophthora infestans isolates and late blight resistance test (IPO-C, 90128, EC1,Pic99189 and DHD11).

13. Detached leaf assays of transgenic potatoes obtained by marker-assisted transformation with single R gene constructs. Non transformed Atlantic or Bintje were susceptible to four P.infestans isolates. Rpi-vnt1- containing transgenic plants were susceptible to EC1 Rpi-sto1- containing transgenic plants were susceptible to pic99189. RESULT

14. It carries a high potential for generating plants with environmental and economic benefits that may be necessary for meeting the global need for a more efficient and sustainable crop production. ( Kushalappa et al ., 2016) Future developments regarding the creation and commercialization of cisgenic and intragenic crops will depend on willingness to pertain less stringent regulation. (Ahmad et al ., 2020). It would be especially helpful to the developer and seed companies. It would provide an additional tool for crop improvement to the breeder and thus increase the production of cisgenic and intragenic crops. Future Trends

15. Reference Chibage , F. C., Nyoni, M., Murashiki , T. C., Samukange , V. C., Muzerengwa , R., Mahuni , C and Savadye , D. T. (2022). Cisgenesis and intragenesis : innovative tools for crop improvement. In  Cisgenic Crops: Potential and Prospects  (pp. 43-65). Cham : Springer International Publishing. Moradpour , M and Abdullah, S. N. A. (2017). Cisgenesis and Intragenesis as new strategies for crop improvement.  Crop Improvement: Sustainability Through Leading-Edge Technology , 191-216. Singh, V., Singh, S., Shikha, K and Kumar, A. (2018). Cisgenesis a sustainable approach of gene introgression and its utilization in horticultural crops: A review.  Int. J. Curr. Microbiol . Appl. Sci ,  7 , 5002-5009. Vasudevan, S. N., Pooja, S. K., Raju, T. J and Damini, C. S. (2023). Cisgenics and intragenics : boon or bane for crop improvement.  Frontiers in Plant Science ,  14 , 1275145.

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