Uses of Double Beam Spectrophotometer

A UV spectrophotometer is used to determine the absorption of light from a sample and can be used as a detector for HPLC. A sample is placed in the UV/VIS beam and absorbance versus wavelength is measured. A double beam spectrophotometer utilizes two beams of light: a reference beam and a sampling beam that passes through the sample. Some Double Beam UV spectrophotometer have two detectors that allow the two beams to be measured at one time.

1. USES OF
DOUBLE BEAM
SPECTROPHOTOMETER
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2. About Us
A UV spectrophotometer is used to determine the
absorption of light from a sample and can be used
as a detector for HPLC. A sample is placed in the
UV/VIS beam and absorbance versus wavelength is
measured. A double beam spectrophotometer
utilizes two beams of light: a reference beam and a
sampling beam that passes through the sample.
Some Double Beam UV spectrophotometer have
two detectors that allow the two beams to be
measured at one time.
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3. Device
This device employs two
light beams, thus the
name “double beam”
spectrophotometer
Reference beam: To measure lamp
energy, this beam passes through the
reference standard.
Sample beam: To reflect sample to
reflect sample absorption.
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4. Applications and Uses of
Double Beam
Spectrophotometers
DNA ANALYSIS
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WAVELENGTH SCANNING
PROTEIN ANALYSIS
QUANTITATIVE ANALYSIS
KINETICS
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5. Key
Operating
Principles
ABSORBANCE
DISPERSION OF LIGHT RAYS
THE VISIBLE SPECTRUM
SCATTERING OF LIGHT RAYS
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6. Effective
Analysis
For effective analysis of the diverse
biological specimens, the double beam
UV/visible spectrophotometer is used.
Measurements of samples are also
performed using the electromagnetic
spectrum. The processes of dispersion,
reflection, and refraction all occur in
spectrophotometry instruments, which
further aids in the taking of analytical
data.

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