VZ notes

Attuluri Vamsi Kumar, MLT, SMAS, Galgotais Unversity.

1. Module-5
Topic name
Varicella Zoster
V-Z
Sub code: BMLT5001
Sub Name: Virology and mycology
Department:Department of MLT, SMAS
Faculty:A. Vamsi Kumar
Designation : Assistant professor
Sem-V

2. SYLLABUS OVERVIEW
Unit-1 Mycology 8 hours
Introduction to medical mycology, basic concepts about superficial and deep mycoses taxonomy , classification &
general characteristics of Various medically important fungi.
Unit-2 Fungal infection 8 hours
Names of fungi & diseases caused by them; superficial mycoses, candida, dermatophytes, opportunistic fungi,
subcutaneous mycoses
Unit-3 Virology 7 hours
Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques
used in virology,
Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)
Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arboviruses, Measles Mumps, Rubella, HIV, COVID-19
Unit-5 DNA Viruses 8 hours
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella Zoster, Hepatitis, Adeno virus
Unit -6 Next generation PCR and it’s modrenapplications 8 Hours
PCR and Next genrationDNA Secquencingtechniques to identify viral genome: History of PCR, Pricipleof PCR, Stages
of PCR, procedure of PCR, Concept of optimization of PCR, Applications of PCR, Advancements in PCR technology, DNA
sequecingtechniques, Methods of DNA Sequencing, Next generation DNA sequencing techniques

3. Unit-4 RNA Viruses 9
hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies,
Arboviruses, Measles, Mumps, Rubella, HIV, COVID-19
Unit 4

4. Unit-5 DNA Viruses
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella
Zoster, Hepatitis, Adeno virus
Unit -5Unit -5

5. Families of viruses
DNA Viruses
HAP3
Herpes viridae
1.Alpha –HS1, HS2, VZ
2.Beta –CMV
3.Gama -EBV
DNA “HAS” the potential
ii. Adenovirus
iii. Small pox
iv. Hepatitis
H

6. General points
1.Introduction
2.Classification
3.Morphology(General properties)
•Size
•Shape
•Symmetry
•Capsomers
•Genome
•Envelop
•Special viral proteins
4.Susceptibility & Resistance
5.Isolation / Cultivation -Animal/tissue/cell
culture
6.Pathogenesis+ mode of transmission +
Mechanism + incubation period
7.Signs & Symptoms+ Complications
8. Laboratory diagnosis
•Sample collection (antemortem + Post
mortem )
•Transport
•Direct microscopy
•Culture / isolation of virus
•Cytopathic effects
•Inclusion bodies or other tests
•Immunofluorescence tests
•Molecular tests
•Ag detection tests
•Detection of viral specific antibodies
9. Prophylaxis(Vaccination)
•Active immunization
•Passive immunization
10. Treatment
1.I
2.C
3.M
4.S&R
5.Cul
6.Patho
7.Signs
8.LD
9.Px
10.T

7. INTRODUCTION

8. Introduction

9. Chicken Pox
(Varicella)
Shingles
(Zoster)

11. Herpes
Viruses
Alpha Beta Gama
HS1, HS2, EBV
Herpes Simplex -1
Herpes Simplex -2
Varicella Zoster
VZ
CytomegaloVirus
CMV
Epstein Barr virus
EBV

12. Morphology
1.Size –100-200nm
2.Shape –Irregular
3.Symmetry –Icosahedral –162
capsomers
4.Genome-dsDNA
5.Envelop –present
6.Special structures –Tegument
membrane

13. Morphology
1.Size –100-200nm
2.Shape –Irregular
3.Symmetry –Icosahedral -–162
capsomers
4.Genome-dsDNA
5.Envelop –present Special
structures –Tegument
membrane

14. Varicella

15. Stages of skin lesions

18. Pathogenesis

19. Clinical Symptoms

20. Laboratory Diagnosis

21. Lab diagnosis

22. Prophylaxis