What is prom

PROM an organic source of chemical DAP

1. “PROM" : The alternatives of DAP,SSP,MAP Nirmal kumar M.Ttech (biotechnology) R.B.S Engg . Tech . Campus Bichpuri agra Project Guide:- Dr. V. K Srivastava HOD , R.B.S engg . Tech . Campus Bichpuri Agra

2. introduction:- What is PROM? Benefits of PROM PROM manufacturing process Specification of PROM Different forms of PROM Objectives Material and method Identification of bacterias

3. What is PROM? Phosphate Rich Organic Manure (PROM) is a green chemistry of phosphate fertilizer. A value-added product produced by co-compositing various organic wastes with high grade rock phosphate in fine size. A variety of organic materials such as FYM, vermicompost dung, crop wastes, press mud from sugar industry, solid wastes from fruit juice industry, oil cakes. Ministry of Agriculture and Cooperation has now pproved PROM as fertilizer in 2012 under Fertilizer Control Order (FCO)

4. Why we use “ PROM” The world today consumes around 150 million tones of high grade phosphate mineral, 90% of which goes into the production of chemical phosphatic fertilizers. Government of India used to spend substantial amount as subsidy to make DAP, MAP and SSP available to Indian farmers at affordable costs During 2010 and 2011 the expenditure of subsidy on DAP sky rocketed to ‘120,000 corer of rupees. This trend continues in one form or the other. Today 50Kg of DAP costs around Rs 1,273/‐ despite heavy subsidy which used to be Rs 600/‐ around the year.

5. Benefits of PROM Acts as alternative to DAP and makes soil soft and enriched with nutrients for long time Provides phosphorus to the second crop planted in a treated area as efficiently as the first Can be produced using acidic waste solids recovered from the discharge of biogas plants, thus makes environment sound Due to organic manure presence, leaching and runoff is prevented Increase the soil water holding capacity.

6. PROM manufacturing process 1. Collect FYM/ vermicompost /anaerobic digest sludge (minimum 2-3 month old )And rock phosphate 2. Blend the material – particle size less then 4 mm (operating temp 30-35 c optimum, 60 maximum) 3. Microbial culture used bacillus megatherium var phosphaticum for phosphate solublising size of inoculum 3%-5% Mixed in bioreactor for 7-10 days 4. Azotobactor microbes culture 3-7 % inoculums use for nitrogen fixing in soil. (This setp is also take 5-10 days ) 5 . After all this activity we pack fertilizer in required bag and store in dry place

7. PROM powder form

8. PROM granules form

9. Specifications of PROM PROM as per FCO are as follows:   [1] Particle Size = 90% passing through 4 mm [2] Total organic carbon = minimum 7.9% [3] Total Nitrogen as N = 0.4% [4] Total P2O5 = 10.42% [5] C:N ratio = less than 20:1 [6] pH of solution = maximum 6.7 [7] conductivity = 8.2 [8] Moisture maximum = 25% [9] Bulk Density (g/cm3) = less than 1.6

10. Field trials of PROM Effect of PROM on yield and net gains in Groundnut crop in comparison with chemical fertilizer as control.   Different land yield

11. objective “To isolate the PROM producing bacteria and process optimization ”

12. Azotobactor bacteria Size -1.5-2.0 μ m Gram negative White in colour ,round shape,chain forming, Aerobic, Growth temp-28±3 Growth time 4-7 days

13. Bacillus megatherium Cell morphology- Rod shape,dimeter 5-7 µm ,cell length -10 µ m,Motile , gram positive Colony morphology – circuler,entire,opaque,white Growth condition- temp- 32 c optimum,pH-7 Growth condition-Aerobic, days- 4-7

14. Azotobactor bacteria identiffication by gram staining method

15. Bacillus megatherium bacteria identification by gram staining method

16. Azotobactor bacteria identiffication by congo red indicator

17. Bacillus megatherium bacteria identification by congo red indicator

18. Result The study of PROM (phosphate rich organic manure)producing bacteria’s name Azotobactor or Bacillus Megatherium is present in the PROM fertilizer we identified those bacteria’s by congo red and gram staining method.

19. THANK YOU