Proteus Vulgaris Microbiology
Proteus vulgaris #12
The importance of identification of a certain microorganisms can range between a life threatening
diseases to a creation of certain antibiotic. Understanding the principals of living microbes and
identifying my unknown bacteria through numerous biochemical and metabolism tests, with the
outmost confidence, Proteus vulgaris had the precise qualifications. The point of this report is to
further explore the identification of my unknown bacteria by revealing the results of the experiments
and comparing them to the other six known bacteria: Micrococcus luteus, Staphylococcus aureus,
Staphylococcus epidermidis, Alcaligenes faecalis, Escherichia coli, and Proteus vulgaris that were
used in the lab, as well as comparing and ... Show more content on Helpwriting.net ...
(Harley, 2011, pp. 102 103) Sucrose and lactose serve as a fermentable carbohydrate sources which
promote its growth of fecal forms and provide color change differentiation. (Harley, 2011, p. 104)
Therefore, if E. coli is being platted on the EMB, after plate s incubation period, it should produce a
green metallic sheen on the agar due to the bacteria being a strong fermenter. My unknown bacteria
tested positive for growth, and agar was fermented reddish burgundy in color. Subsequently, the
unknown bacteria was later inoculated with a sterilized loop into the liquid tryptic soy broth and
incubated at an appropriate temperature. Its results were used for proper identification of turbidity,
spots and flocculation. (BD™ Tryptic Soy Broth (TSB), 2008) The results of the unknown were
cloudiness and some settlement on the bottom of the tryptic soy liquid. The next step was conducted
to find out if all the bacteria, as well as the unknown culture, required oxygen for growth, varying
from an aerobic environment, where bacteria needs oxygen to grow, to facultative anaerobic
environment, where bacteria will grow either with or without oxygen but better in its presence. All the
bacteria, along with the unknown, were separately inoculated and tested. My unknown culture results
tested positive for growth in facultative anaerobic environment. In the next sequence of lab
experiments, the results of unknown bacteria were determined by glucose fermentation,
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