BIOFORTIFICATION OF GRAIN SORGHUM FOR ENHANCING ZINC AND IRON OUTLINE OF POST GRADUATE RESEARCH WORK SUBMITTED TO : Directorate of Research & Dean P.G. Studies Anand Agricultural University, Anand - 388110 SUBMITTED BY : Pal Anjali D. M.Sc.(Agronomy) 1 st sem Reg.No . 2010123029 2
Sr. No. Role Name & Designation 1 Major guide Dr. C. H. Raval Assistant Professor College of Horticulture AAU, Anand. 2 Minor guide Dr. N. J. Jadav Professor & Head, Department of Soil Science & Ag. Chemistry B. A. College of Agriculture AAU, Anand. 3 Member Dr. Vinod B. Mor Assistant Professor & Head, Department of Agronomy College of Agriculture, AAU, Jabugam 4 Member Dr. A. D. Kalola Professor & Head, Department of Agricultural Statistics B. A. College of Agriculture AAU, Anand ADVISORY COMMITTEE 3
INTRODUCTION Scientific name : Sorghum bicolor (L.) Moench . Family : Poaceae Origin : Africa Chromosome no : 2n = 20 Sorghum is one of the most important tropical crop grown in India and best adopted in the region of low rainfall. Sorghum grain contains about 10-12% protine, 3% fat, 70% carbohydrate,vitamins and mineral salts which are essential for effective growth of human life. Sorghum is the world’s fifth-most important cereal crop after rice, wheat, maize and barley. Sorghum is considered as camel crop because of resistance to drought. 4
Maharashtra and Karnataka account for 57.25% of Sorghum (Jowar) produced in India. MP, Tamil Nadu, Rajasthan & Andhra Pradesh are the next major contributing states. India ranks fifth in total sorghum production with 4.23 million tonnes grown in an area of 3.90 million hectare in 2021-22. It is a key cereal crop globally and act as a source of grain , animal feed, pasturage, fiber, fuel, bioethanol, alcoholic beverage as well as building materials. India Area (Lakh ha ) 39.21 Production (Lakh tonnes ) 42.30 Yield (kg/hectare) 1128 Cont….. 5
6 Area “00” ha Production “00” MT Yield (Kg/ha) Gujarat 427.59 575.37 1345.60 Surat 96.11 113.79 1183.99 Tapi 75.70 140.39 1854.50 Porbandar 67.20 90.79 1351.04 Source: Directorate of Agriculture, Gujarat
OBJECTIVES 7 To study the effect of soil and foliar application of zinc and iron on growth, yield, nutrient content and quality of grain sorghum. To study the effect of soil and foliar application of zinc and iron on their availability in soil.
REVIEW OF LITERATURE 8
Sr. No. RESEARCHER SOURCE RESULT 1 Madanu akhila et al., (2021) Department of Agronomy, Sam Higginbottom University of Agriculture, Technology & Sciences, Prayagraj , Uttar Pradesh, India Journal of Pharmacognosy and Phytochemistry 10(2):1292-1295 Application with RDF + 0.5 %ZnSO 4 Foliar spray at 25 and 45 DAS found significantly higher in plant height (164.53 cm ),dry weight (44.67 g/plant), crop growth rate (23.00 g/m2/plant), more number of effective tiller/plant and ears/plant and maximum number of grains /ear (844.38) and grain yield (4433.33 kg/ha) were recorded as compared to other treatments. 2 Veeranagouda R. Maganur and V.S.Kubsad (2020), College of Agriculture, Dharwad, Karnataka, India J. Farm Sci., 33(2): (188-191) Among all other treatments, application of ZnSO 4 +FeSO 4 @15.00 kg/ha each enriched with FYM recorded higher grain yield (4,132 kg/ha), fodder yield (9.95 t/ha). Same treatments recorded higher Zinc (23.43 mg/kg) and Iron (33.89 mg/kg) content in grain. Growth and Yield indices like plant height (232.7 cm ), total dry matter production (218.9g/plant), test weight (3.02 g),number of leaves/plant (7.7) were also higher with same treatment. REVIEW OF LITERATURE 9
Sr. No. RESEARCHER SOURCE RESULT 3 Brajkishor Prajapati and Kewalanand (2019) College of Agriculture, GPBU & T, Pantnagar-263145, India International Journal of Current Microbiology and Applied Sciences 8(12): 1693-1699 RDF (NPK 120:60:40 kg/ha ) in combination with ZnSO 4 15 kg/ha + FeSO 4 15 kg/ha as soil application showed maximum growth attributes characters (plant height, L:S, stem diameter ), dry matter content, crude protein content, green fodder yield of 613.36 and 624.63 q/ha , dry fodder yield of 170.39, 177.21 q/ha as well as crude protein yield 16.65 and 17.44 q/ha, net and B:C ratio during 2012 and 2-13, respectively followed by RDF +ZnSO 4 25 kg/ha. 4 A.H.Anilkumar and V.S.Kubsad (2017) Dharwad, Karnataka, India J. Farm Sci., 30(4): (547-549) Results of the study revealed that soil application of RDF+ Enriched FYM 1 (i.e. 50 kg FYM/ha + 3.75 kg ZnSO 4 /ha FeSO 4 /ha) recorded significantly higher grain yield (4287 kg/ha), fodder yield (7.51 t/ha), gross returns (211924 rupee /ha ),net return ( 85702 / ha ) and BC ratio (3.50) over control, recommended dose of fertilizer and recommended package of practices. The growth and yield parameter viz., leaf area (48.08 dm 2 /plant ),grain weight (62.33 g/plant ) and test weight (37.77 g ) were higher with same treatment. 10
Sr. No. RESEARCHER SOURCE RESULT 5 J S Mishra et al.,(2015) Directorate of Sorghum Research, Telangana, Hyderabad, India Indian Journal of Agricultural Sciences 85(5): 721 Experiment concluded that sorghum cultivar Phule Maulee with soil application of ZnSO 4 + FeSO 4 each at 50 kg/ followed by foliar application (0.50% + 1.0%) at 45 DAS along with recommended dose of fertilizer (80:40:40 kg NPK/ha) could be used to produce micronutrient ( Fe and Zn) rich post-rainy sorghum and to improve the micronutrient nutritional status of farmers. 6 Anuradha Pawar et al., (2015) College of Agriculture, Parbhani. V. N. Marathwada Krishi Vidyapeeth, Parbhani, Maharashtra, India Indian J. Dryland Agric. Res. & Dev. 2015 30 (2): 74-83 The treatment T 7 i.e. RDF + soil application of 15 kg ZnSO 4 + 15 kg FeSO 4 /ha recorded highest % N (0.65), % P(0.49) and K (0.82) in fodder ,whereas highest N, P and K in harvested grains was noted as 1.55 , 0.88 and 0.41 kg /ha , respectively. Total uptake of major also recorded as nitrogen (50.79kg/ha), phosphorus (31.85 kg/ha ) and potassium (32.29kg/ha). 11
Sr. No. RESEARCHER SOURCE RESULT 7 P. B. Adsul et al.,(2014) College of Agriculture, Latur, Maharashtra, India BIONFOLET 11(2 C):578-582 Application of RDF +15 kg ZnSO 4 + 15 kg ZnSO 4 /ha significantly increased sorghum grain yield to 20.58 q/ha. application of RDF +15 kg ZnSO 4 +15 kg FeSO 4 also increased N uptake (50.79 kg/ha), P uptake (31.85 kg/ha ), K uptake (32.29 kg/ha ) in kharif sorghum. 12
EXPERIMENTAL DETAILS 1 Title of experiment Biofortification of grain sorghum for enhancing zinc and iron 2 Location College Agronomy Farm, B. A. College of Agriculture, AAU, Anand 3 Season and Year Summer, 2024 4 Experimental design Randomized Block Design 5 Replication 03 6 Treatments 12 7 Total plots 36 13
EXPERIMENTAL DETAILS 8 Plot size Gross Plot : 3.6 × 5.00 m Net Plot : 2.70 × 4.00 m 9 Seed rate 15-20 kg/ha 10 Spacing 45 cm 11 Method of sowing Drilling 12 Crop and variety Summer sorghum, GJ 43 13 Fertilizer 90-40-40 NPK kg/ha 14 Time of sowing Fortnight of February 14
15 T 1 : 10 kg ZnSO 4 Soil application T 2 : 10 kg FeSO 4 Soil application T 3 : 10 kg ZnSO 4 /ha as basal + 0.5 % ZnSO 4 foliar spray at 30 and 45 DAS T 4 : 10 kg FeSO 4 /ha as basal + 0.5 % ZnSO 4 foliar spray at 30 and 45 DAS T 5 : 5 kg ZnSO 4 + 5 kg FeSO 4 /ha as basal + 0.5 % ZnS0 4 + 0.5% FeSO 4 foliar spray at 30 and 45 DAS T 6 : 20 kg ZnSO 4 Soil application T 7 : 20 kg FeSO 4 Soil application T 8 : 20 kg ZnSO 4 /ha as basal + 0.5 % ZnSO 4 foliar spray at 30 and 45 DAS T 9 : 20 kg FeSO 4 /ha as basal + 0.5 % ZnSO 4 foliar spray at 30 and 45 DAS T 10 :10 kg ZnSO 4 + 10 kg FeSO 4 /ha as basal + 0.5 % ZnSO 4 + 0.5 % foliar spray at 30 and 45 DAS T 11 : Control (RDF) T 12 : Absolute Control TREATMENT DETAILS
OBERVATION TO BE RECORDED (A) Growth attributes Plant population per meter row length at 15 DAS Plant height (cm) at 30, 45, 60 and at harvest Number of leaves at 30, 45, 60 and at harvest (B) Yield attributes Grain yield in kg/ha Straw yield in kg/ha (C) Quality parameters Dry matter (%) Crude protein (%) in grain and straw HCN content at 30, 45 and 60 DAS Zn and Fe content in grain and straw after harvest (D) Soil analysis Initial soil status ( EC, pH, OC%, available N, P, K ( kg/ha) Initial and after harvest Zn and Fe content 16