Anticancer Potential of Viola Odorata against Lung Cancer.pptx

screenin of Lavandula angustifolia and petals of Rosa damascena for anticancer potential.

1. SYNOPSIS SEMINAR Name : Taniya Abid Reg No. : MSH-2023-686 Program: Masters in Molecular Biology and Biotechnology Minor : Biochemisrty Division of Plant Biotechnology Faculty of Horticulture Sher -e-Kashmir University of Agriculture Science & Technology of Kashmir

2. Advisory Committee   Major Advisor: Dr. Khalid Z. Masoodi Assistant Professor Division of Plant Biotechnology Members: Dr. Mudasir Mir Assistant Professor Division of Plant Biotechnology Member from Major Subject Dr. Khalid Hussain Bhat Assistant Professor Division BSH, FOH Member from Minor Subject Dr . Imran Khan Associate Professor Division of Agri. Statistics Member from Supporting Subject Dr. Asima Amin Assistant Professor Division of Vegetable Science Dean’s Nominee

3. Proposed Title Anticarcinogenic and Anti invasive Properties of Viola odorata Against Lung Cancer

4. Viola odorata is commonly known as Sweet Violet, English Violet, Common Violet, or Garden Violet and Gulbanafsa in Hindi. V. odorata is a native of Mediterranean countries and Asia Minor. From old ages it has been grown in gardens, and now it has spread to most of Europe. V. odorata is indigenous to India and found in Kashmir, Himachal Pradesh, and Kumaon hills. V. odorata is commonly used as remedy for coughs and sore throat, hoarseness and tonsillitis. V. odorata is valued as an expectorant, diaphoretic, antipyretic, antibacterial, diuretic and as a laxative, in bilious affections. Kingdom Plantae Class Magnoliopsida Order Violales Family Violaceae Genus Viola Species Odorata INTRODUCTION Singh A et. al. 2018.

5. Lung cancer Background Lung Cancer Prevalence Lung cancer is one of the most commonly diagnosed cancers worldwide, with a significant impact on global health. Limited Treatment Options Current treatment options, such as surgery, chemotherapy, and radiation therapy, have limited effectiveness and often come with severe side effects. Need for New Strategies The limitations of existing treatments highlight the urgent need for the development of new, more effective and safer therapeutic approaches for lung cancer. Addressing the limitations of current lung cancer treatments is crucial, driving the exploration of alternative strategies like investigating the anticancer potential of natural compounds such as Viola odorata.

6. Why Viola odorata? Anti-inflammatory properties Viola odorata has been reported to possess potent anti-inflammatory activities, which could be beneficial in reducing inflammation associated with lung cancer. Antioxidant properties The plant extract of Viola odorata has shown strong antioxidant capabilities, which may help in neutralizing the oxidative stress and damage caused by lung cancer cells. Anticancer properties Previous studies have suggested that Viola odorata exhibits anticancer effects against various cancer cell lines, including lung cancer cells. This study aims to further investigate the anticancer potential of Viola odorata against lung cancer. Ayshah et. al. 2020.

7. OBJECTIVES Assessment of anti proliferative and anti invasive activity against lung cancer cell line A-549. Expression analysis of proinflammatory genes involved in lung cancer.

8. Summarized flowchart of the protocol Collection of Viola odorata Soxhlet extraction Cell culture studies Metabolite Profile CFU assay, WHA, MTT RT expression of Lung Cancer genes GCMS Data acquisition and analysis

9. MTT Assay MTT assay is colorimetric assay based on the reduction of MTT, a yellow tetrazole, by mitochondrial enzymes to form a purple formazan that is measured by spectrophotometric methods. MTT not only determines cell count and cell proliferation, but it can also be used for determining the cytotoxicity of various substances that would either inhibit or stimulate cell growth and propagation.

10. Wound Healing Assay Wound healing assay, also known as a scratch assay, is a common in vitro technique used to study cell migration . This assay is widely used in cell biology to investigate how cells move and interact, and it is particularly relevant in research focused on cancer, tissue regeneration, and cellular response to injury. CONTROL TREATED 0-hr 48-hr 72-hr 24-hr

11. Colony Formation Unit assay(CFU) A Colony Forming Unit (CFU) assay is a widely used technique in microbiology and cell biology to measure the number of viable cells in a sample, particularly those that can grow and form colonies. This assay is often used to evaluate the efficacy of antimicrobial agents, the viability of cell cultures, and the ability of cells to proliferate. .

12. Statistical Analysis The data obtained from the study will be subjected to analysis of variance (ANOVA) and/ OR Student’s t-test whichever applicable. All assays will be performed in triplicates reproducibly. Graphpad Prism software will be used for the analysis

13. Expected Outcome Pave way for discovery of new anticancer molecules against lung cancer. molecules can be utilised to develop nutraceuticals as well as pharmaceutical drugs against lung cancer

14. Thank You