Biosafety Course (Bio hazards BSL measures)

Biosafety Course

1. Dr SourishGhosh
Senior Scientist,
Infectious Diseases & Immunology Division,
CSIR-Indian Institute of Chemical Biology, Kolkata
An Introduction to Biosafety

2. Hazardous Agents in lab
Any potential source or substance that can cause harm to
humans, animals, or the environment either by itself or
through interaction with other factors.
Types of hazardous agents:
1-Chemical
2-Radiological
3-Electrical
4-Biological
Dr. Sourish Ghosh, CSIR-IICB 2

3. Dr. Sourish Ghosh, CSIR-IICB 3
Chemical Hazards
How can it harm you?
Chemical spillage on your skin.
Inhalation of chemical.
Ingestion while pipetting.

4. Dr. Sourish Ghosh, CSIR-IICB 4
Radiological Hazards
Types of Radiation:
Ultraviolet radiation (UV lamps).
Infra-red radiations (Infra-red heat lamps).
Lasers.
X-ray or Gamma Rays.
Principle of radiation safety:
The distance between the radiation source and personnel exposed
should be increased. Usually doubling the distance from the source
will reduce the radiation exposure by a factor of 4.

5. Dr. Sourish Ghosh, CSIR-IICB 5
Electrical Hazards
Courtesy: UCLA Environment, Health & Safety

6. Dr. Sourish Ghosh, CSIR-IICB 6
Biological Hazards
Samples having pathogen
contamination transmitted to your body
by inhalation, injection, or ingestion.
Bacteria (Salmonella sp., Shigella
sp.)
Viruses (Hepatitis C & B, HIV).
Fungi (Candida, Mucor).

7. Dr. Sourish Ghosh, CSIR-IICB 7
Biological Hazards
Sources: Respiratory secretions, CSF,
blood, urine, tissues from infected animals,
and fluids from infected arthropods.
Primary Hazards: Direct contact of skin or
mucous membranes with infectious
materials, accidental parenteral inoculation,
ingestion, and exposure to aerosols.
Special Hazards: Cultures have been more
commonly associated with infection than
clinical materials and infected animals.

8. Biosafety
•The application of knowledge, techniques, and equipment to
prevent personal, laboratory, and environmental exposure to
potentially infectious agents or biohazards.
•Objectives of studying biosafety:
•to reduce the potential exposure of the laboratory worker,
persons outside of the laboratory, and the environment to
potentially infectious agents
Dr. Sourish Ghosh, CSIR-IICB 8

9. Dr. Sourish Ghosh, CSIR-IICB 9
Biosafetylevels
Lowest Safety Level
Not known to cause
diseases in adult human
Non-pathogenic microbe
Moderate Danger
If inhaled, swallowed
or injected to skin.
Influenza, Dengue
Severe/ Potentially
Lethal
Highly transmissible
known to cause diseases
M. Tuberculosis,
SARS-Cov2
Highest Safety Level
Life threatening disease
whose treatment
is unknown
Ebola

10. Dr. Sourish Ghosh, CSIR-IICB 10
Courtesy: Environmental Health & Safety-University of California, Riverside

11. Dr. Sourish Ghosh, CSIR-IICB 11
Biosafetylevel 1
(minimum risk)
Laboratory Types: Standard teaching and research laboratories.
Laboratory Practices Requirements: Standard Good Laboratory
Practices (PPE), hand washing, and decontamination of work surfaces.
Safety Equipment Required:
Open bench , no need for Biological safety cabinets (BSCs).

12. Dr. Sourish Ghosh, CSIR-IICB 12
Biosafetylevel 1
(minimum risk)
3
2
1

13. Dr. Sourish Ghosh, CSIR-IICB 13
Biosafetylevel 2
(moderate risk)
Laboratory Types: Primary healthcare diagnostic and research
laboratories.
Laboratory Practices Requirements: PPE, restricted access to
laboratory areas.
Safety Equipment Required:
1-Open bench + hood
2-Autoclave
3-Strict dealing with waste
4-stick biological symbols

14. Dr. Sourish Ghosh, CSIR-IICB 14
Biosafetylevel 2
(moderate risk)

15. Dr. Sourish Ghosh, CSIR-IICB 15
Biosafetylevel 2
(moderate risk)

16. Dr. Sourish Ghosh, CSIR-IICB 16
Biosafetylevel 2
(moderate risk)

17. Dr. Sourish Ghosh, CSIR-IICB 17
Biosafetylevel 3
(high risk)
Laboratory Types: Research and diagnostic lab.
Laboratory Practices Requirements: PPE, restricted access to
laboratory areas, special clothing, directional air flow.
Safety Equipment Required:
Same of level 2.

18. Dr. Sourish Ghosh, CSIR-IICB 18
Biosafetylevel 3
(high risk)

19. Dr. Sourish Ghosh, CSIR-IICB 19
Biosafetylevel 3
(high risk)
How to enter BSL-3?

20. Dr. Sourish Ghosh, CSIR-IICB 20
Biosafetylevel 3
(high risk)
Workflow in BSC in BSL-3
Experiment
Discard

21. Dr. Sourish Ghosh, CSIR-IICB 21
Biosafetylevel 3
(high risk)
How to exit BSL-3?

22. Dr. Sourish Ghosh, CSIR-IICB 22
Biosafetylevel 4
(life threatening)
Laboratory Types: Dangerous pathogen unit
Laboratory Practices Requirements: PPE, restricted access to
laboratory areas, special clothing, air lock entry, shower exit, special
waste disposable.
Safety Equipment Required:
1-Autoclave
2-Class III hood
3-Filtered air

23. Dr. Sourish Ghosh, CSIR-IICB 23
Biosafetylevel 4
(life threatening)

24. Dr. Sourish Ghosh, CSIR-IICB 24
Biosafetylevel 4
(life threatening)

25. GLP
Good lab practices

26. Dr. Sourish Ghosh, CSIR-IICB 26
GLP
1-Do not eat or drink in the lab and don’t use fridge for food
2-Report any accident (spill, breakage, etc.) or injury (cut, burn, etc.) to
the teacher immediately, Do not panic.
3-Never attempt unauthorized experiments
4-Keep work places clean and free of unwanted chemicals, biological
specimens.
5-Wear Gloves , lab coat, mask and glasses.
6-Tie hair and use closed shoes
7-Do not touch , taste, or smell any chemicals.

28. 8-Do not use an unlabeled bottle.
9-Work only with materials once you know their
flammability, reactivity, toxicity, safe handling and
emergency procedures.
10-Never pipette by mouth; use mechanical transfer
devices.
11-Do not mix chemicals in the sink.
12-If a chemical was splashed in your eye(s) or on your
skin, immediately flush with runningwater for at least 20
minutes.

29. 13-Walk DON’T run in the lab.
14-Keep exits and passageways clear at all times.
15-Turn of gas, water before leaving.
16-Wash your hands thoroughly before leaving the laboratory.
In case of emergency, contact your lab safety officer or the
institute’s occupational health center.