botany presentation on environmental.pptx

botany presentation

1. Phytochemical Analysis and Establishment of Hydroponic System for Selected Wild Vegetable Name:- Shavitri Kushwaha 620

2. INTRODUCTION The seed of it is used as a poultice for broken bones and the root of it is used to treat gonorrhea.  It has been reported in modern scientific analysis that it has anti-depressant activity,  anti-diabetic properties,  anti-inflammatory activity,  antibacterial property,  anti-malarial 

3. Seeds Inflorescence Flowers Leaves and Inflorescence

4. Estimation of chlorophyll They are bound loosely to proteins but are readily extracted in organic solvents such as acetone or ether.

5. Method

6. •Calculate the amount of chlorophyll present in the extra using this  following equations.  1)mg Chlorophyll (a/g)=12.7 (A 663)-2 (As 645) x V÷1000×W 2)mg Chlorophyll (b/g)=22.9 (A645)-4.68(A663)×V÷1000×W 3)   mg total Chlorophyll=20.2(A645)+8.02(A663)×vV÷1000×W

7. Result:-

8. Estimation of crude fiber

9. Materials

10. Methods

11. • Calculation :-

12. Antioxidant activity

13. •The dried plant material was powdered by using a grinder.  •It was extracted using maceration method with ethanol 70% as a solvent.  •The maceration method has been used for 3 x 24 hours with solvent replacement every 24 hours.

14. Test sample Methanol DPPH 10ul 990ul 2ml 30ul 970ul 2ml 50ul 950ul 2ml 100ul 900ul 2ml 500ul 500ul 2ml 1000ul 0ul 2ml Blank 1000ul 2ml Incubate for 30 min in dark Absorbance was read at λ518 nm wavelength using spectrophotometer.

15.   Thin layer chromatography:-

16. Procedure

17. 254nm   366nm   IN UV Chamber

18. Qualitative analysis:- •The total content of each of these phytochemicals is usually determined by carrying out phytochemical tests (based on colorimetry) for each of these secondary metabolites. •The secondary metabolite groups considered in this study were alkaloids, saponins, polyphenols, flavonoids, and tannins. •They exhibit significant pharmacological and biological properties and are hence used for medical purposes

19. Estimation of Tannins   The total Tannin content in a Amaranthus spinosus plant sample was  milligram to per gram of weight of the sample using the equations y=0.007x-o.043;with =0.992                  The Tannin contents in the Amaranthus plant sample was found out 0.9mg/g

20. Estimation of Total Phenolics :-  The total Phenols content in a Amaranthus spinosus plant sample was  milligram to per gram of weight of the sample using the equations y=0.005x-0.234;with =0.941                   The Phenol contents in the Amaranthus plant sample was found out 0.08mg/g 

21. Estimation of Flavnoid The total Flavonoid content in a Amaranthus spinosus plant sample was  milligram to per gram of weight of the sample using the equations y=0.016x-0.111;with =0.951                  The Flavonoid contents in the Amaranthus plant sample was found out 0.6mg/g

22. Hydroponic •Hydroponics is a type of horticulture and a subset of hydroculture which involves growing plants, usually crops, without soil, by using water-based mineral nutrient solutions in aqueous solvents. •They are a rich source of vitamins, essential amino acids and have rich iron content. •. It is a well-known fact that green vegetables work like wonders in our diet.

23. Hydroponic  System

24. Methodology

25. DAY 1

26. DAY10

27. Harvesting Amaranth from Hydroponics:

28. Conclusion

29. Thank you