Collection and transport of urine specimen Arman ali M.Sc final year
INTRODUCTION Collection Techniques :- Specimens should be collected during the acute (early) phase of an illness (or within 2 to 3 days for viral infections) and before antimicrobials, antifungals , or antiviral medications are administered. Patient information to match & lavel properly :- Name Identification number Collection Information Date and time Total volume Collection duration (i.e., 24-hour)
Information For The Nursing Staff And Clinicians Should Include The Following Safety considerations Selection of the appropriate anatomical site and specimen Collection instructions, including the type of swab or transport medium Transportation instructions, including time and temperature constraints Labeling instructions, including patient demographic information (minimum of two patient identifiers) Special instructions, such as patient preparation Sterile versus nonsterile - collection devices
URINE SPECIMEN Clean- voided midstream (CVS) Container :- Sterile, screwcap container ; Containers that include a variety of chemical urinalysis preservatives may also be used. Patient Preparation :- Females : clean area with soap and water, then rinse with water; hold labia apart and begin voiding in commode; after several mL have passed, collect midstream. Males : clean glans with soap and water, then rinse with water; retract foreskin; begin voiding in commode; after several mL have passed, collect midstream .
From Straight catheter: container Sterile, screw-cap container or urine transport tube with boric acid preservative Patient Preparation In catheterized patients , urine should be collected from the catheter tube (after disinfecting ) Insert catheter into bladder; allow first 15 mL to pass; then collect remain. Special Instructions – urine not collect from uro bag. Transportation to Laboratory Unpreserved ≤1/2 h/RT- Preserved ≤ 24 h/RT ; Storage Before Processing 24 h/4°C
From Suprapubic aspiratation : Suprapubic aspiration (SPA) is a sterile procedure that allows for the sampling of uncontaminated urine in patients. It is considered the gold standard for collecting urine for urinalysis in children it is recommended for patients in coma or infants. container - Sterile , screwcap container or anaerobic transporter Patient Preparation -Disinfect skin. Special Instructions Needle aspiration above the symphysis pubis through the abdominal wall into the full bladder. Storage -Immediately/RT
PROCEDURE I nsert the needle (attached to the syringe) perpendicular to the skin at 1-2 cm superior to the pubic symphysis (the suprapubic crease level) in the midline : Aspirate gently after the needle pierces the skin. Aspirate as you insert, as well as when you withdraw the needle.