Cultivation of microalgae in Fresh & Marine water

Microalgae Cultivational Types and it's Growth media

1. VIVEKANANDHA
COLLEGE OF ARTS AND SCIENCE FOR WOMEN
Veerachipayalam, Sankari, Salem, Tamilnadu
DEPARTMENT OF MICROBIOLOGY
SUBJECT : MICROALGAL TECHNOLOGY
Subject Incharge
Dr. R. DINESHKUMAR
Assistant Professor
Department of Microbiology
viaas, Sankari.
Presented by :
B. K. KAVYADHARSHINI
I - Msc. Microbiology
Department of Microbiology
Viaas, Sankari.

2. TOPIC :
CULTIVATION OF FRESH
AND MARINE MICROALGAE -GROWTH
MEDIA

3. 1. Introduction
2. Microalgal Cultivation
3. Importance & Requirements
4. Methods of Cultivation
5. Applications
6. Growth Media
7. Components
8. Consideration
9. Applications of Growth
media
10. Conclusion
List of Contents :

4. Introduction :
◾️Algae are simple autotropic organisms, mostly found
in aquatic Environments.
◾️It is a plant like organisms.
◾️It can be unicellular or Multicellular.
◾️Also present in various colors.

5. Microalgal
Cultivation :
??????Microalgae cultivation is the process of growing
and maintaining microalgae under controlled natural (outdoor) or
artificial (indoor) conditions.
?????? It involves providing suitable growth media, light,
temperature, pH, nutrients, and aeration so that they can multiply,
photosynthesize and produce useful metabolites.

6. Process:

7. Macroalgae
Microalgae

8. Biofuel production
Food and feed
Pharmaceutical & Neutraceutical
value
Environmental Benefits
Industrial applications
Scientific & Research Importance
Importance of Microalgae :

9. 1. Light
2. PH
3. Temperature
4. Nutrients
5. Water
6. Carbondioxide
7. Mixing and Aeration
8. Carbon supply
10. Sterility and Contamination
Ctl
General Requirements :

10. ◾️Autotropic cultivation
◾️Heterotropic cultivation
◾️Mixotropic cultivation
Methods of Cultivation:
Types:

12. Autotropic cultivation
:
??????Autotrophic cultivation refers to the process of
growing organisms, such as microalgae, that produce their own
food through photosynthesis or chemosynthesis.
??????They use energy from light, carbon dioxide & organic
substances to synthesize organic compounds.
?????? Eg : Cyanobacteria, Nitrogen fixing & sulfur bacteria.

13. ??????Heterotrophic cultivation refers to the process of
growing organisms, such as microalgae or bacteria, that require
organic substrates (e.g., sugars, organicacids) as a source of energy
and carbon.
Hetetropic cultivation :
??????Eg - Chlorella, Tetraselmis.

14. Mixotropic Cultivation :
?????? Mixotrophic cultivation refers to a mode of growth
where organisms, such as algae or bacteria, can simultaneously
use both autotrophic and heterotrophic metabolisms
Eg : Euglena & few Bacterias
Euglena :

16. ??????Biofuel production
??????Pharmaceuticals
??????Food & Neutraceuticals
??????Aquaculture and animal feed
??????Waste water treatment
??????pigments & cosmetics
??????Bioplastics & Biofertilizers
Applications:

17. Growth Media :
◾️Also called Culture Media.
◾️It is a substance or mixture that supports the
growth of Microorganisms, Cells & Tissues
◾️It provides all the Nutrients, Salts, other
necessary components for its Growth, Reproduction & Survival.

18. It plays a vital role in algal culture &
contains the balanced mixture of nutrients.
Growth Media:
▪️BG - 11 Medium
▪️BBM - Bold's Basal Medium
▪️Chu's Medium - Chu 11 & chu q3
Fresh Water Medium

19. They are specialized solutions that supports the growth
of marine microalgae. It contains salts, nutrients, and vitamins for
its growth factor.
Marine Water Medium
◾️f/2 Medium
◾️Guillard's Medium

20. Components of Growth Media:
Carbon, Hydrogen, Phosphorus, Sulphur, Nitrogen, Potassium,
Magnesium, Calcium.
Micronutrients
Iron, Boron, Manganese, Silicon, Zinc, Copper,
Molybdenum,Cobalt.
Macronutrients

21. Factors Affecting Media
Preparation :
Nutrient
availablity
PH Levels
Temperature
Salinity
CO2
Concentration

22. Applications:

23. REFERENCES
:
John D.M., Whitton B.A., Brook A.J.(2002).The
Freshwater Algal Flora of the British Isles. Cambridge: Cambridge
UniversityPress.1- 118.
Kitaya Y.H.,Azuma M.,Kiyota (2005).Effects of
Temperature,CO,/O, Concentrations andLight Intensity on Cellular
Multiplication ofMicroalgae Euglena gracilis. Adv. Space
Res 35(9): 1584-1588.

24. THANK
YOU...!