dr veera kapha ketu rasa.ppt description

Dr veera kaphaketu rasa

1. Anti-Histamic activity of
Kaphaketu rasa- An Invivo
study

Presenter;
Dr.Veera Jogur
1
st
year PG Scholar
Dept.of Rasashastra &
bhaishajya kalpana
AMV Hubli.

2. INTRODUCTION
•Kaphaketu Rasa is very popular organo-mineral preparation used for Vata-Kaphaja
Pratishaya, Kasa-Swasa and other Pranavaha Sroto dusti vikaras and for many other
diseases.
•The Kaphaketu Rasa has various methods of preparation and clinical applications.
•Hence how to assess its quality and standard is a question in this scientific and
technologically advanced era.
• High prevalence rate of allergic disorders ranging between 25% to 50% during the life
time of persons their ever increasing incidence of severity worldwide are a matter of
concern.
•Kaphaketu Rasa may be the answer for all afore said concern
•The present study is designed to establish its effects more scientifically by evaluating:
Anti histaminic property of Kaphaketu Rasa experimentally in particular
respiratory allergic disorder.
Pharmaceutics, by adopting standard Operative Procedures.
Physico chemical study; to support experimental study.

3. AIMS AND OBJECTIVES
•Establish conceptual theme on organo-mineral preparation w.s..r. to
Kaphaketu Rasa.
•SOP and SMP will be developed for Kaphaketu Rasa.
•Physico-chemical standards was developed for kaphaketu Rasa.
•The other criterion of selection is based on to establish scientifically
its efficiency as an anti histaminic activity through experimental
study.

4. REVIEW OF LITERATURE
•Formulation Review:
Kapha Ketu Rasa mentioned in Kapha Roga Chikitsa Prakarana of Rasendra Sara
Sangraha is selected.
•Ingredients of Kapha Ketu Rasa
Tankana, Pippali, Shankha Bhasma and Vatsanaba in equal parts and Ardraka
Swarasa for bhavana.
•Method of preparation of Kapha Ketu Rasa
Shanka Bhasma, Shodita Tankana, Shodita and Vastragalita Vatsanabha and Pippali
choornas are to be homogenously mixed and Bhavana with Ardraka Swarasa should
be given for 3 times. Then the dried drug is further powdered. The prepared
compound drug Kapha Ketu Rasa is then ready for dispensing at a dose of 2 Ratti.
•Indications of Kapha Ketu Rasa:
Kasa, swasa, Peenasa, netraroga, Galaroga, Karnaroga, Galagraha, Danta Roga.

5. Drug Guna Karma
Matr
a
Anupana
TANKANA The rasa of Tankana is Katu,
Kshara, veerya is ushna,Amla
Vipaka,and having ushna,
Rooksha, Teekshna and Sara
guna.
Vata Kaphaghna Lekhana, Rechana, Agnikara, Vishadoshahara,Hrdya,
Dravi, Swarnaraupya Shodhana, Akhiladhatuvedhi, Balya,Stri puspajanana,
Mudha garbha Pravartaka, Kapha vislesana,vividha vrana nasaka, Varnya,
Arthavajanak.
Tankana (Borax) produces cold feeling and diuresis, induces
mensturation and uterine contractions, acts as antacid, antimicrobial,
local depressant and anti inflammatory.
1-2
Ratti
.
Madhu,
Ghrita
PIPPALI Sushka pippali is Katu rasa,
Ushna Veerya, having Laghu,
Snigdha, Teekshna guna and
Madhura vipaka, Whereas Ardra
pippali is Madhura rasa, Sheeta
veerya with guru and Snigdha
guna.
Dry pippali allievate vata and Sleshma dosha, but fresh pippali
vitiate kapha dosha. Both varieties are Deepaka Amapachaka
Vrishya, Rasayana, Rechaka.
Rogaghnata :Udara, Pliharoga, Jwara, Kushta, Prameha,
Gulma, Arshas, Shoola, Amavata, Trishna, Krimi.
Piper longum or piperine shows to possess bio availability
enhancing activity with various structurally and
therapeutically diverse drugs
0.5
– 1
gm
Madhu
SHANKHA The rasa of Shankha bhasma
is Kshareeya and Madhura,
its Veerya is sheeta ,guna is
Laghu and Vipaka is Katu.
Pitta Kaphahara, Grahi, Balya, Lekhana, Agni Deepaka,
Vishadoshahara, Graha Dosha nasaka, Varnya, Chakshushya,
Netrapushpahara.
Rogaghnata:Grahani, Visha, Tarunya pidika, Agnimandhya,
Parinama Shoola, Amlapitta, Kshaya, Netraroga, Swasa,
Kapharoga, Gulma.
2
Rati
Nimbu
swarasa
or
Ushnod
aka

6. Drug Guna Karma
Matr
a
Anupana
VATSANABHA
Vatsanabha is Madhura in
rasa with Laghu, Rooksha,
Teekshana, Vyavayi and
Vikasi Gunas with Ushna
veerya and Katu Vipaka. Its
prabhava is Rasayana.
Vatakaphahara, Jwarahara, Jangama Vishahara, Madakari,
Rasayana, Kustaghna, Deepana, Balya, Bruhmana.
Rogaghnata: Jwara, Jangama visha, Kusta, Madhumeha, swasa,
Kasa, Amavata, Shotha, Plihodara, Agnimandya, Vata
rogas,Timira, Bhagandara, Arshas, Vrana.
It is remedy for acute catarrah of nasal and facial, acute
pharyngitis, ulcerated tonsillitis pneumonia, acute bronchitis,
laryngitis, pleurisy, acute catarrhal pneumonitis,
1/16
to
1/8
ratti
Madhu
ARDRAKA
Ardraka is Katu Rasa, Guru,
Rooksha, Teekshna guna,
Ushna Veerya and Madhura
Vipaka.
Vatakaphahara, Deepana, Bhedana, Vrshya, Ruchya Hrdya
pachana.
Rogaghnata: Shoola, Amavata, Adhmana, Atisara, Sleepada,
Kasa, Swasa, Hrdroga, Sopha, Arshas, Hika, Vibanda,
Raktapitta, Pandu, Vrana, Jwara, Kusta, Agnimandya, Vamana,
Shotha.
1-
2gm
s
Madhu

7. PRATISYAYA
Nidana sevana vata + kapha & pita located at shiras pratisyaya
Poorvarupa (Primodial symptoms)
•Feeling of heaviness of head
•Sneezing
•Bodyache
•Horripilations
•Many other secondary disease like
fever, loss of taste, etc.
ALLERGIC RHINITIS
Dusta pratishyaya lakshana
• Nose getting wet oftenly
• Nose gets dry oftenly
• Nasavarodha and get decongestion
• Smell turns very foul
• Loss of smell, taste
• The condition is krichra saadhya
This is a disorder in which there are episodes of nasal
congestion, watery nasal discharge and sneezing. It may
be seasonal or perennial.
Clinical features
In the seasonal type there are frequent sudden attacks of
sneezing, with profuse watery nasal discharge and nasal
obstruction,accompanied by smarting and watering of
the eyes and conjunctional injection.In the perennial
type the symptoms are similar, but more continuous and
generally less severe.
Parameters of the study
•Kshavatu (Sneezing)
•Nasasrava (Nasal Discharge)
•NasavaRodha (Nasal Blockage )
•GalaGraha (Throat Irritation)
“
प्रतिक्षणम्श्यायतेइतिप्रतिशायाय
:”

8. Pharmaceutical Review
1 2 3 4 5 6
Wt of tubers after 24 hrs of
steeping
Qty. of cow’s
urine added
Qty.of cow’s urine at the
end of 24 24hrs of steeping
Cow’s urine
absorbed
pH before/ after 24
hrs of steeping
Specific gravity before/after
24 hrs of steeping
500gm / 720 gm 1.5 ltr 750 ml 750 ml 7.6 / 5.22 1.012 / 1.035
720gm / 760 gm 1.7 ltr 1.250 ml 450 ml 7.5 / 5.36 1.015 / 1.026
760 gm / 780 gm 1.750 ltr 1.500 ml 250 ml 7.2 / 5.85 1.015 / 1.018
Result :- Weight of Ashodhita Vatsanabha = 500 gm
Weight of Shodhita Vatsanabha = 430 gm
Net loss = 70 gm
Followed by swedana in Godugda for 3 Hrs
No. Of days required for Shodhana of Vatsanabha 6 days
Showing observation of Vatsanabha Shodana
VATSANABHA
Wt of Shodhita Vatsanabha : 400 gms
Wt of Shodhta Vaatsanabha choorna: 380 gms
Wt loss: 20 gms
Pippali Choornikarana
Results:
Wt of Pippali: 500 gms
Wt of Pippali choorna: 480 gms
Wt loss: 20 gms

9. Ardraka Swarasa Nirmana
Results:
Wt of Ardraka -2 kg
Qty of swarasa- 800 ml
Net loss- 1200 gm.
SHANKHA [Strombus giges] SHODHANA using Kanji
pH Recording during Shodhana Procedure
Time 0.5hr 1hr 1.5hrs2hrs 2.5hrs3hrs
pH 4.2 4.59 5.23 5.34 5.38 5.40
Result
Weight of Shankha before Shodhana - 600gm
Weight of Shankha after Shodhana - 585gm
Net loss - 15gm
MARANA OF SHANKHA
Temperature Recorded during 1
st
Gajaputa
Atmospheric Temperature – 34˚ C
Duration of temperature recorded – 7.5 hrs
Maximum peak temperature – 846˚ C
Time taken for base to peak temperature – 100 min
Time taken for peak to base temperature – 5.50 min
Weight of Shodhita shankha – 550 gms
Weight of marita shankha after 1
st
Gajaputa – 510 gms
Net loss - 40 gms
Temperature Recorded during 2
nd
Gajaputa.
Atmospheric Temperature – 36˚ C
Duration of temperature recorded – 7.40hrs
Maximum peak temperature - 837˚ C
Time taken for base to peak temperature - 90min
Time taken for peak to base temperature – 6.10min
Weight of Marita Shankha before 2
nd
Gajaputa – 510 gms
Weight of Marite Shankha after 2
nd
Gajaputa - 490 gms
Net loss - 20 gms

10. Preparation of Kaphaketu Rasa.
Results:
After I Bhavana
Weight of homogeneous mixture of ingredients of Kaphaketu Rasa 400 gms
Amount of Ardraka swarasa 800 ml
Weight of dried Kaphaketu Rasa 520 gms
Net gain in the weight of Kaphaketu Rasa 120 gms
It took 8 hours to complete 1
st
Bhavana.
II Bhavana
Weight of I Bhavita Kaphaketu rasa 520 gms
Freshly prepared Ardraka swarasa 750 ml
The same method was followed.
III Bhavana
Weight of dried Kaphaketu rasa 600 gms.
Freshly prepared Ardraka Swarasa 500 ml.
Weight of Kaphaketu rasa before 3
rd
Bhavana 600 gms.
Weight of Kaphaketu rasa after 3
rd
Bhavana 640 gms
Net increase in weight 40 gms.
It took 4½hours to complete 3
rd
Bhavana
Thus prepared Kaphaketu rasa was filled in gelatinous
capsules at the dosage of 125mg and stored.
The same parameters were applied for the preparation of Kaphaketu rasa another two times.
So the sample A,B,C of Kaphaketu rasa were prepared and sent for analytical study.
•Material colour of the mixture appeared like peethabha,
•Weight of Kaphaketu rasa before 2
nd
Bhavana 520 gms.
•Weight of Kaphaketu rasa after 2
nd
Bhavana 600 gms.
•Net increase in the weight of Kapha ketu rasa 80 gms.
•It took 6 hours to complete 2
nd
Bhavana.

11. ANALYTICAL REVIEW
Organoleptic Characters
a) Colour- Ash
Gray.
b) Smell- Faint.
c) Touch-
Smooth.
d) Taste-
Pungent. Showing Quantitative Estimations
Drug Physicochemical Parameters Solubility
Foreign
Matter
Ash Value Acid insoluble
Ash Value
Water Alcohol
Ashodhita Vatsanabha Nil 4.52% w/w 1.04% w/w 27.57w/w15.34%w/w
Shodhita Vatsanabha Nil 4.38%w/w 0.64%w/w 25.46%w/w13.52%w/w
Shodhita Shankha Nil 96.91%w/w 53.80%w/w - -
Shankha Bhasma Nil 36.59%w/w 1.43%w/w
Pippali Nil 9.02%w/w 1.67%w/w 53.3%w/w 8.75w/w
Ardraka Nil 4.08%w/w 0.68%w/w 30.56%w/w 13.96w/w
Kaphaketu Rasa Nil 32.42%w/w 2.08%w/w 52.94%w/w -

12. Showing physical constants of
KaphaKetu Rasa.
Sl NoPhysical
constants
Kaphaketu rasa
Sample A
Kaphaketu rasa
Sample B
Kaphaketu
rasa
Sample C
1
pH 7.48 7.52 7.50
2
Specific
Gravity
1.002 1.006 1.004
3
Moisture
content
1.94 1.96 1.92
4
Total Ash
45.64% 45.68% 45.62%
5
Acid
Insoluble
Ash
44.32% 44.38% 44.36%
6
Water
Soluble Ash
18.64% 18.68% 18.66%
7
Water
Insoluble
Ash
27% 29% 30%
Shows Phytochemical constituents of
Kaphaketu Rasa
Sl.
No.
Organic
Constituents
Kaphakethu rasa
1Alkaloids Present
2Carbohydrates Absent
3Tannins Absent
4Steroids Present
5Triterpenoids Present
6Flavanoids Present
7Protiens Absent
8Saponins Present
9Carotenoids Absent

13. Showing Retention factor Values of Different components.
Samples
R
f values
Formulatio
n
(Sample 1)
0.10
(violet)













0.14
(violet)
0.27
(violet)
-
0.45
(violet)
-
0.56
(violet
)
Pure
Vatsanabha
(Sample 2)
- - - - -
0.46
(p.blue)

-
0.55
(violet
)
Impure
Vatsanabha
(Sample 3)
0.12
(violet)

-
- -
0.34
(violet)
0.45
(p.blue)
0.51
(red)
0.56
(violet
)
Shunthi
(Sample 4)
0.10
(f.yellow)
0.14
(f.yellow
)
0.17
(f.yellow)
0.27
(blue)
0.34
(p.blue)
0.46
(p.blue) -
0.56
(violet
)
Pippali
(Sample 5)
0.10
(f.yellow)
0.14
(f.yellow
)
0.17
(f.yellow)
0.27
(blue)
0.34
(p.blue)
0.46
(p.blue) -
0.56
(violet
)
Track1 Mixture of pure vatsanabha,
shunthi and pippali 20μl
Track 2 Pure vatsanabha 20μl
Track 3 Impure vatsanabha 20μl
Track 4 Shunthi 20μl
Track 5 Pippali 20μl
Track 6 Mixture of pure vatsanabha,
shunthi and pippali 10μl
Track 7 Pure vatsanabha 10μl
Track 8 Impure vatsanabha 10μl
Track 9 Shunthi 10μl
Track 10 Pippali 10μl

14. Shows Action and Characteristics of different Phyto Constituents in Drugs.
Class Action Characteristics
Alkaloids Analgesic, Bacteriocidial, Antiviral, Anti
tumour, Antispasmodic
Bitter taste, Nitrogen
containing Basis
Tannins Soothing relief, Regenerates Skin, Anti-
inflammatory, Diuretics, Anti malarial
Unpleasant taste, Tans leather
Steroids Anti inflammatory, Analgesic, Immuno
suppressants of histamine
Bitter in Taste.
TriterpinoidsAntiseptic, Analgesic, Carminative,
Stimulant
Bitter in Taste
Flavonides Anti oxidants, Anti Carcinogenic, Anti
tumour, Anti Microbial.
Water soluble, Super Anti
oxidant, free Radical
scavenger.
Saponnins Expectorant, Cough suppressants,
hemolytic activity.
Bitter Taste, foaming
property.

15. ANIMAL EXPERIMENT
Results and Discussion:
The KaphaKetu Ras at 23.25mg/1.5kg, p.o., significantly extended the latent period of PCD as
compared with the control [ Tab. 1], following exposure to histamine aerosols on day 3, 4 and 5
respectively. The graph was shown as the onset of dysponea (sec) and the KaphaKetu Ras
compared with the control groups (n=5) for 5days as shown in [ Fig. 1].
Groups Pre-convulsion dysponea* (sec)
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5
Control 157.4±17.60158.8±19.18152.4±19.48146.8±16.92137.6±13.56141.6±15.71
Extracts(mg/kg)154.8±18.03141.6±25.24134.4±22.37222.6±22.26*306.0±31.00*
*
340.0±23.22**
*
P value 0.9203 0.6022 0.6727 0.0266
KaphaKetu Ras Activity on histamine induced bronchospasm in guinea pigs:

16. Animal Experiment
Guinea pigs
Guinea pigs
Anti-Histaminic
Chamber
K.K.Ras Feeding K.K.Ras Feeding

17. DISCUSSION
Discussion on SOP and SMP of Kaphaketu Rasa:
On observing different physical constants of three samples of Kaphaketu Rasa we
can infer that Pharmaceutics followed in the preparation of Kaphaketu Rasa is well according
to classics standard operative procedures and standard manufacturing process. We can claim
that the prepared drug was of higher standards.
Discussion on Phyto constituents:
Qualitative identification of Phyto constituents in Kaphaketu Rasa revealed that it
contains Alkaloids, Steroids, Triterpenoids, Flavonids and Saponins. As Kaphaketu Rasa is
composed of useful wide above phytoconstituents it can be said that it is effective in
management of curative properties of Alkaloids Anti septic and Anti microbial action and
Immuno stimulant properties of Triterpenoids, the steroidal anti inflammatory synergies the
action of Kaphaketu Rasa.

18. Discussion on Animal Experiment:
Kaphaketu Rasa Activity on Histamine induced Broncospasm in guinea pigs was
conducted to establish its Antihistaminic Activity. Kaphaketu Rasa prolonged latent period of
Pre-convulsion Dyspnoea (PCD) following histamine aerosol, peaks were observed in 4
th
day
and 5
th
day of the study; PCD latent period increased from 3
rd
day onwards; this may be
suggestive of anti histaminic activity of Kaphaketu Rasa.
Discussion on HPTLC:
The choromatography of mixture shows the presence of major
components of sample (II) Shudha Vatsanabha in large and some components of
Shunthi and Pippali are also present in low percentage, may be because of
Kaphaketu Rasa is treated with highly volatile solvent media.

19. CONCLUSION
Khalviya Rasayana is a unique scientific pharmaceutical method to bind phyto ingredients to the metallo
mineral ingredients, without direct agni paka. Hence all the volatile and Non-volatile active components
are kept intact with formulations. More over with less instrumentation and least technology by process of
Bhavana, Mardhana. There by all ingredients will form a monomolecular compact form i.e. each molecule
of ingredients of Kaphaketu rasa treated with Ardraka swarasa and whole mass of Kaphaketu Rasa
becomes highly potent.
Phyto ingredients of Kaphaketu Rasa were analysed physically and chemically, Phyto chemicals of
Kaphaketu Rasa were analysed; HPTLC was also studied to know standard qualities of ingredients. All the
above tests were within the standard limit. Hence proving its safety and genuinity.
Three samples of KaphaKetu Rasa prepared according to the classics showed no much changes in
physical constants. Hence the procedure followed is of standard one.
Animal experiment was conducted on Histamine induced Bronchospasam in Guinea pigs; and anti
histaminic activity of Kaphaketu Rasa is statistically significant.
Kaphaketu Rasa acts on Kapha and Vata; as Shotahara, Kledamalahara, Krimigna, Vatanulomaka,
Tridosha Shamaka; acts as analgesic, anti inflammatory, anti biotic, anti histaminic activity. In total
Kaphaketu Rasa acts as immune Suppressant of Histaminic activity also as a Rasayana. Proving to be the
best, effective, safe medication for Pratishaya (Allergic Rhinitis).