Endospores_Dr Jagadisha T V _CSD 2nd pptx

Formed inside the parent vegetative cell.
➢ Endospores are highly durable dehydrated cells,
which can survive extreme heat, lack of water,
freezing and exposure to many toxic chemicals
and radiation.
➢ Endospores also called as “resting cells”.

1. BACTERIAL ENDOSPORES Dr. Jagadisha T.V.,  M.Sc., PGDGT., PhD Assistant Professor  Department of Life Sciences (Genetics) Kristu Jayanti College (Autonomous)   (Reaccredited  A++ Grade by NAAC with CGPA  3.78/4)   K. Narayanapura, Kothanur Post, Bangalore - 560 077 Karnataka, INDIA. Ph.-No: 8892698143/9449442521, 

2. Spores Endospore; ➢ Formed inside the parent vegetative cell. ➢ Endospores are highly durable dehydrated cells, which can survive extreme heat, lack of water, freezing and exposure to many toxic chemicals and radiation . ➢ Endospores also called as “resting cells ”. o Exospore ; ➢ Formed outside the vegetative cell by budding at one end of the cell. ➢ The Exospore do not contain dipicolinic acid. ➢ They can resist desiccation and heat

3. Bacterial Endospores Endospores are the resisting structures formed by bacteria for survival during adverse environmental condition. Endospores differ significantly from the vegetative and normal functioning of cells. Endospores provide resistance against Heat Drying Low nutrient conditions Radiation Osmotic pressure Various chemical disinfectants

4. SPORE PROTEINS Dipicolinic acid Located in the core Calcium – Dipicolinic acid complexes reduces water available and helps to dehydrate spores . Interculates into the DNA and stabilizes it to heat denaturation . Small Acid Soluble Proteins (SASPs) Bind to the DNA in the core and protect it from damage . Function as a carbon and energy source when forming vegetative cells to spore cells .

5. The shape of the spore is an IDENTIFYING CHARACTERISTIC Swelled vs. Not swelled spore Bacterial cell spore Bacterial cell

6. LOCATION OF THE SPORES Central, Sub- Terminal, and Terminal spores

7. Endospore Staining

8. Endospore formation : When certain essential elements like carbon ,nitrogen and phosphorus are limited or completely depleted or when water is unavailable certain gram (+) bacilli from specialized resting cells called endospores . These endospores are highly durable dehydrated cells which can survive extreme heat, lake of water, freezing and exposure to many toxic chemicals and radiation . The genera of bacteria giving rise to endospore