geneticallymodifiedorganism-220916094813-6891e260.ppt

gmo

1. ‘GENETICALLY MODIFIED
ORGANISMS’
DR. RACHANA CHOUDHARY
Asstt. Prof. Department of Microbiology
Shri Shankaracharya Mahavidyalaya, Shri Shankaracharya Mahavidyalaya,
Junwani,Bhilai (Durg).Junwani,Bhilai (Durg).

2. Genetic modification is the process of altering the properties
of cells in an organism by changing the genetic makeup of the
DNA in the nucleus of the cell. This can include deleting or
changing genes, or transferring genes from one organism to
another. When an organism has had these changes made to it,
it will become a genetically modified organism and DNA
fragments are called rDNA and their technique is genetic
engineering.
Such as Bacteria and yeast, plants, fish, and mammals.
INTRODUCTION:INTRODUCTION: - -

3. HISTORY OF GMOHISTORY OF GMO
•The first GMOs were bacteria in 1971.
•Creation of the first recombinant DNA
molecules by Paul Berg in 1972.
•Insulin –producing bacteria were
commercialized in 1982.

4. CONSTRUCTION OF GMOsCONSTRUCTION OF GMOs
Fig- Steps in molecular cloning (modified)

5. EXAMPLES OF GMO’s EXAMPLES OF GMO’s
•In 1994, the Flavr SavrFlavr Savr tomato was introduced as
the first GM food.
•Golden riceGolden rice – enriched rice containing beta-carotene
(Vitamin A).
•Bt cornBt corn – corn containing a chemical normally found
in bacteria (Bacillus thuringiensis).
•Herbicide resistant plants (roundup ready corn).

7. Plants are genetically modified to be:
•Herbicide resistant
•Pesticide resistant
•Insect resistant
•Drought tolerant
•Extreme temperature tolerant
•Have added nutrients, such as vitamins and
minerals
GENETICALLY MODIFIED PLANTSGENETICALLY MODIFIED PLANTS

8. Roundup is a common herbicide
manufactured by Monsanto that
is harmful to weeds and plants
alike For this reason, Monsanto
developed a line of “Roundup
ready” crops that are resistant to the herbicide By inserting gene 5’
‐
enolpyruvylshikimate 3’ phosphate (EPSP) from the bacteria
‐ ‐
Agrobacterium, plants such as corn, soybeans, cotton, and alfalfa
could be made herbicide resistant
HERBICIDE RESISTANTHERBICIDE RESISTANT

9. Corn, cotton, and several other plants have
been genetically modified to be insect
resistant.
Insect resistance in crops is accomplished by
identifying and isolating a gene from the soil
bacterium Bacillus thuringiensis that
produces a toxin called Cry that is toxic to
plant insects.
INSECT RESISTANCEINSECT RESISTANCE

10. Rice, has been genetically modified to be
an improved source of vitamin A.
Biosynthesis of beta carotene in GM rice was accomplished by
‐
inserting phytoene synthase (psy) gene from daffodils and
phytoene desaturase (ctrI) gene from the bacteria Erwinia
uredovorainto rice DNA
The additional beta carotene produced by the endosperm (rice
‐
grain that is eaten by the humans) gives it a characteristic yellow
or golden hue Because of this the vitamin enriched GM rice is also
‐
known as “golden rice”
GM PLANTS WITH ADDED NUTRIENTS ‐RICEGM PLANTS WITH ADDED NUTRIENTS ‐RICE

11. Strawberries, which are a good source of vitamin C,
have been genetically modified to provide 3 times
as much vitamin C.
A gene in the strawberry plant called GalUR gene codes for an
enzyme that converts a protein in the plant to vitamin C.
A similar gene is found in the thale cress Arabidopsis thaliana.
Researchers created a DNA plasmid using the A. thaliana gene and
the bacteria Agrobacterium and inserted into the strawberry plant to
over express GalUR gene and produce 3 times as much vitamin C
‐
GM PLANTS WITH ADDED NUTRIENTS ‐STRAWBERRIESGM PLANTS WITH ADDED NUTRIENTS ‐STRAWBERRIES

12. Currently, researchers around the world are working at
creating and perfecting:
• Drought resistant wheat, corn, and rice
• Salt tolerant tomatoes
• Frost resistant strawberries
• Heat tolerant beans such as kidney, red, black,
and pinto beans
• Carrots that produce a vaccine against hepatitis B
MORE GM CROPSMORE GM CROPS

13. GENETICALLY MODIFIED ANIMALSGENETICALLY MODIFIED ANIMALS
•Genetically modified
mammals are an
important category of
genetically modified
organisms. Ralph L.
Brinster and Richard
Palmiter developed
the techniques
responsible for
transgenic mice, rats,
rabbits, sheep, and
pigs in the early
1980s.

14. The FDA Center for Veterinary Medicine(CVM) regulates
genetically altered animal products
Currently no transgenic animals have been approved for human
consumption
Transgenic animals have been approved for use as biopharm
animals (for producing drugs and hormones) and they produce
such products as milk and wool.

15. •Production of animals with specific traits much
quicker than with traditional breeding methods
Results in –
•Better quality with increased milk production
and wool production, Increased growth rates
Efficient production of pharmaceuticals,
nutritional supplements, and hormones.
•Most pharmaceuticals are produced from the
milk of goats, cows and sheep Included are such
drugs as Insulin, Growth hormone.
BENEFITS OF TRANSGENIC ANIMALSBENEFITS OF TRANSGENIC ANIMALS

16. •Experts estimate that producing therapeutic protein
using traditional methods cost approximately $300
‐
$3,000 per gram.
•In contrast, using a transgenic goat to produce the
protein in milk costs approximately $20 $105 per
‐
gram.
•Transgenic hen eggs are even cheaper, costing
approximately $.10 $.25 per gram of protein
‐

17. GENETICALLY MODIFIED MICROBESGENETICALLY MODIFIED MICROBES
•Bacteria were the first organisms to be modified in the
laboratory, due to their simple genetics. They are important in
producing large amounts of pure human proteins for use in
medicine.
•Genetically modified bacteria are used to produce the protein
insulin to treat diabetes. Similar bacteria have been used to
produce clotting factors to treat haemophilia and human
growth hormone to treat various forms of dwarfism.

19. EXAMPLES OF MICROBES AS GMOEXAMPLES OF MICROBES AS GMO

20. USES OF GMOUSES OF GMO

23. CREATION OF SUPERBUG FOR CREATION OF SUPERBUG FOR
DEGRADING XENOBIOTICSDEGRADING XENOBIOTICS

24. CHALLENGES #1CHALLENGES #1
•EnvironmentalEnvironmental – possibility of unintended harm to other
organisms:
•potential risk of harm to non-target organisms, e.g. a pest
resistant crop that produces toxins that may harm both crop-
damaging and non crop-damaging insects
•E.g. The pollen of BT corn on milkweed is thought to affect
(slow or kill) the larvae of Monarch butterflies. Further
studies are underway.

25. CHALLENGES #2CHALLENGES #2
•pesticides become less effectivepesticides become less effective as pests become resistant to
modified crops.
•Different varieties and strengths of pesticides will be needed
once weeds have adapted to the existing effective pesticides.

26. CHALLENGES #3CHALLENGES #3
•““Superweeds”Superweeds”
gene transfer to non-target species where
herbicide tolerant plants crossbreed with
weeds potentially creating herbicide resistant
weeds.
Some Western Canadian farmers are calling
Monsanto’s round-up ready canola a
superweed.

27. CHALLENGES #4CHALLENGES #4
•Human health risksHuman health risks
▫introducing a gene into a plant
may create a new allergen or
cause an allergic reaction in
susceptible individuals
▫For example, inserting genes
from a nut into another plant
could be dangerous for people
who are allergic to nuts

28. CHALLENGES #5CHALLENGES #5
•Economic HazardsEconomic Hazards
•Elimination of competition
▫GM seeds are patented (must buy each year)
•This presents problems for poor farmers in both the developed
and developing worlds.
▫Large companies like Monsanto have resorted to suing small
farmers found to be using their seed without paying.
•Suicide seeds
▫Plants with sterile seeds that are infertile are created
▫Farmers are forced to buy seeds every year
•However, some companies have reduced costs or donated GM
seeds to impoverished nations.

29. CONCLUSIONCONCLUSION
Despite the great promise of genetic engineering it also brings
with it potential problem in area of safety, human experience,
potential ecological disruption & biological warfare.
genetically engineered microbes are emerging as a efficient
vehicle to overcome environmental pollutions.

30. THANK YOUTHANK YOU