The quantification of ABA contents was conducted by Wuhan MetWare Biotechnology Co., Ltd. (Wuhan, China), as previously described [27]. Briefly, fifty seeds were rapidly frozen in liquid nitrogen and ground into a fine powder. Subsequently, 50 mg of the powdered sample was utilized for hormone extra...
The quantification of ABA contents was conducted by Wuhan MetWare Biotechnology Co., Ltd. (Wuhan, China), as previously described [27]. Briefly, fifty seeds were rapidly frozen in liquid nitrogen and ground into a fine powder. Subsequently, 50 mg of the powdered sample was utilized for hormone extraction, using a mixture of methanol, water, and formic acid in the ratio of 15:4:1. The combined extracts were then evaporated to dryness under a stream of nitrogen gas and redissolved in 80% methanol. The resulting sample extracts were filtered and analyzed using an LC-ESI-MS/MS system (consisting of an HPLC Shim-pack UFLC SHIMADZU CBM30A system coupled with an MS Applied Biosystems 6500 Triple Quadrupole). The internal standards of 2H6-ABA were employed in this study for accurate quantification.
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INFO8135-Descriptive Analytics-New PPT.pdf
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