Krebs cycle.pptx

This is meant for exploratory purposes.

1. The institute of integrated & honors studies Submitted to :- Submitted by :- Dr. Suhana Rao Amit Kumar Botany Dept.(IIHS,KUK) B.Sc. Medical (180009) BOTANY ASSIGNMENT ON Krebs Cycle 2020-2021

2. Krebs Cycle

3. Krebs Cycle K nown as the Hans Krebs cycle.

4. Krebs Cycle K nown as the Hans Krebs cycle. (After the scientist who discover it in1937.) Hans-Adolf-Krebs (Nobel -1953)

5. Krebs Cycle K nown as the Hans Krebs cycle. (After the scientist who discover it in1937.) Also Citric acid cycle. ( T he molecule Citric acid/ citrate that play central role in cycle.) TCA Cycle (tricarboxylic acid cycle). Hans-Adolf-Krebs (Nobel -1953)

6. Krebs Cycle Krebs Cycle is series of chemical reaction that release stored energy of carbohydrate, fat and protein through the oxidation in the form of NADH and FADH.

7. Krebs Cycle Krebs Cycle is series of chemical reaction that release stored energy of carbohydrate, fat and protein through the oxidation in the form of NADH and FADH. Provide raw material like amino acid, NADH etc.

8. Site of occurrence - Eukaryotic Cell Prokaryotic C ell

9. Site of occurrence - Eukaryotic Cell Prokaryotic C ell Matrix of mitochondria

10. Site of occurrence - Eukaryotic Cell Prokaryotic C ell

11. Some background knowledge about the steps taken before the Krebs cycle-

12. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle

13. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA

14. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA where does it's come from?

15. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis

16. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C 6 H 12 O 6 6C

17. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C 6 H 12 O 6 6C 2 x Pyruvate (3C)

18. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C 6 H 12 O 6 6C 2 x Pyruvate (3C) Pyruvate dehydrogenase complex COA

19. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C 6 H 12 O 6 6C 2 x Pyruvate (3C) Pyruvate dehydrogenase complex COA (2C) CO 2 & NADH

20. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C 6 H 12 O 6 6C 2 x Pyruvate (3C) Pyruvate dehydrogenase complex COA (2C) CO 2 & NADH This shows that how Acetyl CoA is formed. Which is 2C compound and starting point of Krebs cycle.

21. Steps of Krebs cycle - Acetyl-CoA 2C

22. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C

23. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase

24. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate

25. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase 6C

26. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C

27. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase

28. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C

29. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA

30. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA NAD+H + NADH CO 2 α - ketoglutrate dehydrogenase 4C

31. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA NAD+H + NADH CO 2 α - ketoglutrate dehydrogenase 4C Succinate 4C

32. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA NAD+H + NADH CO 2 α - ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ P i GTP

33. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA NAD+H + NADH CO 2 α - ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ P i GTP Fumarate 4C

34. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA NAD+H + NADH CO 2 α - ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ P i GTP Fumarate 4C FAD+ H + FADH Succinate dehydrogenase

35. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA NAD+H + NADH CO 2 α - ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ P i GTP Fumarate 4C FAD+ H + FADH Succinate dehydrogenase M alate 4C

36. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA NAD+H + NADH CO 2 α - ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ P i GTP Fumarate 4C FAD+ H + FADH Succinate dehydrogenase M alate 4C Fumarase

37. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA NAD+H + NADH CO 2 α - ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ P i GTP Fumarate 4C FAD+ H + FADH Succinate dehydrogenase M alate 4C Fumarase

38. Steps of Krebs cycle - Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso -citrate A conitase α - ketoglutrate 6C Isocitrate dehydrogenase NAD+H + NADH CO 2 5C Succinyl CoA NAD+H + NADH CO 2 α - ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ P i GTP Fumarate 4C FAD+ H + FADH Succinate dehydrogenase M alate 4C Fumarase Malate dehydrogenase NAD+H + NADH

39. Results - Summery Reaction of Krebs Cycle- Acetyl CoA + 3 NAD + FAD + GDP + PO 4 + 2H 2 O 2CO 2 + 3NADH + FADH + GTP + CoA

40. Results - Summery Reaction of Krebs Cycle- Acetyl CoA + 3 NAD + FAD + GDP + PO 4 + 2H 2 O 2CO 2 + 3NADH + FADH + GTP + CoA How many energy Produce? In one rotation – 12 ATP ( 3 NADH + 1 FADH + 1GTP) NADH = 3 ATP 1 FADH = 2 ATP 1GTP = 1ATP

41. Results - Summery Reaction of Krebs Cycle- Acetyl CoA + 3 NAD + FAD + GDP + PO 4 + 2H 2 O 2CO 2 + 3NADH + FADH + GTP + CoA How many energy Produce? In one rotation – 12 ATP ( 3 NADH + 1 FADH + 1GTP) NADH = 3 ATP 1 FADH = 2 ATP 1GTP = 1ATP For completion of reaction rotation should be twice So net ATP form - 12 ATP x 2 = 24 ATP

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