PETRI DISH DOCUMENTATION.pptx

Petri Dish experiment

1. GROWING BACTERIA IN PETRI DISH EXPERIMENT GROUP III

2. PRESENTED BY: CASTRO, JEANLYN V. DUCLAYAN, REXANNE FIA MAYEE A. SALIDA, NADENE KATE G. WANAWAN, ROWIE B.

3. THE MAKING BRAINSTORMING ACTION PLAN PREPARATION GET TO KNOW EACH OTHER

4. MATERIALS AGAR POWDER PETRI DISH TIMER COTTON SWABS MEASURING SPOONS MICROWAVE BEAKER BOX TAPE ZIPLOCK BAG FACE MASK HOT WATER LAB GOWN SAFETY GOGGLES SURGICAL CAP SURGICAL GLOVES DISINFECTANTS

5. WHAT IS PETRI DISH EXPERIMENT ALL ABOUT? PETRI DISH EXPERIMENT IS ABOUT GROWING OF BACTERIA IN AN AGAR-FILLED PETRI DISH.

6. PROCEDURE 1. Clean the materials

7. 2. Mix ½ teaspoon agar (about 1.2 grams) with ¼ cup (60 mL) of hot water in a clean, microwave safe container and stir.

8. 3. Place the container in the microwave and bring to a boil for three minutes to completely dissolve the agar. The mixture should be clear with no particles floating around in the solution. Allow the mixture to cool for 3 to 5 minutes before moving on.

9. 4. Fill the bottom half (smaller half) of the Petri dish with the warm agar solution and cover loosely with the top half of the petri dish (it may be necessary to set the lid ajar to allow moisture to escape). Label the petri dish while waiting for solution to cool and harden.

10. 5. Roll a clean cotton swab in your sample area. Be sure to wipe the end of the cotton swab all over the surface to be tested and cover the entire end of the swab with invisible bacteria.

11. 6. Lift the top off the Petri dish and LIGHTLY draw a squiggly line in the agar with the end of the cotton swab.

12. 7. Cover the Petri dish with the top half and use a piece of paper or tape to label the dish with the name of the item you tested. Be careful that when you replace the top of the Petri dish, that there is not excessive moisture. You can place the Petri dish upside down if moisture would drop on your sample. After you have completed the sample and labeled the dish, place your Petri dish in a sealed Ziploc bag

13. HOW DO BACTERIA GROW IN PETRI DISH ? BACTERIA DO GROW IN PETRI DISH BY THE HELP OF NUTRIENT AGAR. NUTRIENT AGAR IS A GENERAL PURPOSE MEDIUM SUPPORTING GROWTH OF A WIDE RANGE OF NON-FASTIDIOUS ORGANISMS. IT TYPICALLY CONTAINS (MASS/VOLUME): 0.5% PEPTONE - THIS PROVIDES ORGANIC NITROGEN AND PROTEIN SOURCE SO THAT MICRO-ORGANISMS CAN GROW. 0.3% BEEF EXTRACT/YEAST EXTRACT - THE WATER-SOLUBLE CONTENT OF THESE CONTRIBUTE VITAMINS, CARBOHYDRATES, NITROGEN AND SALTS WHICH SERVES AS FOOD FOR GROWING BACTERIA.

14. TABLE OF OBSERVATION IN FIVE CONSECUTIVE DAYS

15. WANAWAN’S PETRI DISH MOUTH SWAB SAMPLE DAYS OF OBSERVATION BEFORE DISINFECTANT AFTER DISINFECTANT RESULT/EVALUATION FIRST DAY (WED, SEPT 15, 2 PM) Before: few tiny clear white spot occurred. Visibility of Bacterial colony can be seen by naked eye. After: few tiny circular and rhizoid colony of bacteria occurred. Colony of bacteria spread upward. SECOND DAY (THU, SEPT 16, 2 PM) Before: colony of bacteria multiplied into numerous circular white spot, one spot on the left has a rhizoid form around it. After: the rhizoid part of bacteria in day 1 spread out and filamentous formed bacteria occurred.

16. THIRD DAY (FRI, SEPT 17, 2 PM) Before: bacterial colony spread through out the entire petri dish forming circular and rhizoid. Black particles started occurring in some circular formed bacteria. After: colony of mixed flat and circular creamy-yellowish bacteria started to spread through out the entire petri dish. FOURTH DAY (SAT, SEPT 18, 2 PM) Before: A bit similar to day 3 but few white spots turned as one. After: Some area of the colony becoming thin and translucent. Few white spots turned as one. FIFTH DAY (SUN, SEPT 19, 2 PM) Before: No visible changes on count, only black particles darken a bit. After: Some area became totally translucent, smooth and very thin.

17. DUCLAYAN’S PETRI DISH CELLPHONE SWAB SAMPLE DAYS OF OBSERVATION BEFORE AFTER RESULT/EVALUATION FIRST DAY (WED, SEPT 15, 2 PM) The first day of my observation of my petri-dish reveals a lot of bacteria thriving. There are a lot of bacteria in the (before) image, as you can see. The bacteria colony expanded out in a non-uniform shape, with white being the most prevalent hue. The (after) picture depicts a smaller bacterium. The colony is primarily small, and its hues are mostly white. SECOND DAY (THU, SEPT 16, 2 PM) Before: The second day of my observation of my petri-dish shows a lot bacterium. It has a many tiny colonies and the bacterium was compact each other and have a bubble.   After: As you can see in the image, the bacteria and colonies separate and spread.

18. THIRD DAY (FRI, SEPT 17, 2 PM) Before: In the picture, the bacterium looks like a cotton, they become visible, and it has a color yellow colony.   After: The (after) pictures shows lot of yellow colonies and they become visible. FOURTH DAY (SAT, SEPT 18, 2 PM) Before: The fourth day of my observation reveals many developing colonies. Bacterial colonies have a variety of coloring, with white being the most prevalent and having an irregular form.   After: As shown in the image, the colony expands in a non-uniform way, with several yellow colonies. FIFTH DAY (SUN, SEPT 19, 2 PM) Before: The bacteria continue to develop and become more apparent, as shown in the image, where the white colonies compress each other, and the yellow colonies are in the midst of the white colonies. It appears that white is the most common color. After: Yellow is the most abundant hue in the image, and they also compact each other. The colonies are mainly small, but they become increasingly noticeable as time goes on.

19. SALIDA’S PETRI DISH FLOOR SWAB SAMPLE DAYS OF OBSERVATION BEFORE AFTER RESULT/EVALUATION FIRST DAY (WED, SEPT 15, 2 PM) Before: shows a lot of growing colonies of bacteria. The size of the colonies is tiny and irregular in shape and it spreads out in a non-uniform pattern. The pigmentation is mostly white or creamy white that are not yet fully visible. After: shows a lesser colony. The colonies are mostly white and a creamy white. The colonies are tiny in size and spreads out in a non-uniform pattern. SECOND DAY (THU, SEPT 16, 2 PM) Before: shows that the bacteria multiplied in number and the colonies doubled in size. The pigmentation shows a clearer hue of the white colonies and yellow colonies started to develop. After: The colonies also double in size. The pigmentation is mostly white and circular in shape and the only yellow colonies that is tiny in size developed and it is circular in shape.

20. THIRD DAY (FRI, SEPT 17, 2 PM) Before: The third day of my petri dish shows that the pigmentation of the colonies is becoming brighter and visible to the naked eye. Just like the pale yellow turned to a brighter yellow that is more visible. After: The after-petri dish started to develop more yellow colonies that is tiny and not yet fully visible. FOURTH DAY (SAT, SEPT 18, 2 PM) Before: Fourth day observation shows that more bacteria are developing into a yellow colony and their size is mostly tiny. The white colonies expand, and it spreads out in a non-uniform pattern. After: The after-petri dish shows that the tiny yellow colonies develop a clearer color of yellow. FIFTH DAY (SUN, SEPT 19, 2 PM) Before: Fifth day observation shows that the yellow colonies doubled in size therefore there are more visible. The white colonies continued to expand in a non-uniform pattern, and they are mostly irregular in shape. After: The after-petri dish shows that more bacteria of colonies start developing into white and yellow colonies. The colonies are mostly tiny and circular in shape. They spread out in a non-uniform pattern.

21. CASTRO’S PETRI DISH WATER DIPPER SWAB SAMPLE DAYS OF OBSERVATION BEFORE AFTER RESULT/EVALUATION FIRST DAY (WED, SEPT 15, 2 PM) In day 1, the result is very interesting because in BEFORE (IMAGE) we can see that it have accumulated to form a visible patches and the bacterial colonies appear white in color and are fairly circular that are spread directly into nutrient agar. The right image which is the AFTER (IMAGE) we can only see a few bacterial colonies that have formed in circular shapes on to nutrient AGAR. SECOND DAY (THU, SEPT 16, 2 PM) In days 2, there are numerous visible bacterial colonies that appear in white color and are small circular shape spread directly into nutrient agar in our BEFORE (IMAGE) while in the AFTER (IMAGE) we can see that a lot of growth bacterial colonies were formed Than in DAY 1. In addition, there are yellow in column and translucent circular in shape bacterial colonies are formed.

22. THIRD DAY (FRI, SEPT 17, 2 PM) In DAY 3, as can be seen in the BEFORE (IMAGE) there are plenty of bacterial colonies were formed than day 2 and are spread in small circular shape in white color. Nevertheless, in AFTER (IMAGE) we can see that a black and yellow translucent bacterial colonies were formed directly onto the nutrient agar. FOURTH DAY (SAT, SEPT 18, 2 PM) In day 4, the number of bacterial colonies are still rising, the white small different shapes were prominent in that are formed in the before image . Whereas in the growth of the bacterial colonies gets higher in our AFTER (IMAGE). The small circular shape are gets darker in color than the third day and the yellow and black color circular shapes bacterial colonies got bigger and darker. FIFTH DAY (SUN, SEPT 19, 2 PM) In Day 5, we can see that the growth of the translucent with small different shapes of bacterias are getting higher in the BEFORE petri dish (IMAGE) while in AFTER petri dish (IMAGE) the small shapes of the translucent bacterias colonies are getting higher. We can see also that the white, black and yellow big shape bacterial colonies are still getting bigger and darker.

23. THANK YOU