Practical application of dna technology

DNA

1. Practical Application of DNA Technology

2. What is DNA Technology? DNA t echnology refers to a wide range techniques used to study, improve, create, or manipulate the genetic material.

3. What Are Some Techniques in DNA Technology?

4. You find a healthy person willing to donate some pancreatic cells. Microtubes

5. Refrigerated Centrifuge

6. Restriction Enzyme To isolate the insulin gene from the numerous genes found in the DNA obtained from the cells, the DNA must be cut into many tiny fragments.

7. Gene Amplification You can make many copies of each DNA fragment by gene amplification using a technique polymerase gene amplification.

8. Gel Electrophoresis Placing the DNA fragments on a gel subjected to an electric field. The fragments move at different speeds and separate from each other along the length of the gel. Technique: gene isolation

9. Gene Mapping Finding the exact location of a gene in the 46 chromosome of the human cell.

10. Recombinant DNA Technology You introduce the insulin gene into a bacterium. Bacteria contain circular DNAs called plasmids . Refers to the process of creating a DNA molecule by joining together DNA or segments of DNA obtained from two different species.

11. Gene Cloning Allowing the transformed bacterial cell to reproduce itself. In the process, more bacteria will posses the human insulin gene.

12. Given favorable conditions, the bacterial colony will express the gene by producing insulin.

13. PRACTICAL APPLICATIONS

14. DNA technology has important uses in basic research and potential applications in medicine, industry, agriculture, and forensics. Practical Applications

15. MEDICINE It has been used for diagnosis and treatment of diseases, correction of genetic disorders, and manufacture of hormones and other pharmaceutical products.

16. Newborn Screening Simple test done on infants with 48 hours after delivery. Congenital metabolic disorder Phenylketonuria Congenital hyperthyroidism Congenital adrenal hyperplasia Galactosemia Glucose-6-phosphate dehydrogenase deficiency (G6PD)

17. Gene Therapy Detectible genetic disorders may be corrected through a procedure called gene therapy. Involves: Extraction of a few cell from the patient Addition of functiona l genes in a cells of the patient that carries defective ones Reintroduction of the corrected cells into the patient

18. Immunization To five a vaccine to prevent infection by a diseases.

19. AGRICULTURE Increased milk production in cows. Diseases resistance Insect resistance Herbicide tolerance in crops Delayed ripening of fruit.

20. FORENSICS Tommie Lee Andrew