Presentación Seminario Biología Molecular.pdf.pdf

Seminario Manuela Montoya y Sara Montoya

1. Manuela Montoya Builes
Sara Manuela Montoya Giraldo
Third Semester of Medicine
Universidad Pontificia Bolivariana

2. INTRODUCTION
vagus
definitions, what is
vagus and stroke
stroke
01
02
It involves using a device to stimulate
the vagus nerve with electrical
impulses. When stimulated, electrical
impulses are sent to different parts of
the brain. This alters brain activity to
treat certain conditions.
An ischemic stroke occurs when
the blood supply to a part of the
brain is blocked or reduced.
Another type is a hemorrhagic
stroke. It occurs when a blood
vessel in the brain leaks or
bursts and causes bleeding in
the brain..

3. INTRODUCTION

4. GENERAL OBJECTIVE
Our goal was to develop a minimally invasive,
targeted, and convenient VNS approach to
assess the
impact of VNS and to clarify the relationship
between VNS and MCs in the prognosis of
patients with myocardial
atrophy after acute ischemic stroke.

5. METHODS
2.4 VAGUS NERVE STIMULATION (VNS)
The rats were immobilized with a rat fixator 24 h after MCAO/r, and before VNS.1.
3. The left vagus nerve was punctured with a sterile 25 mm needle, and the needle
handle was connected to a Korean electroacupuncture instrument.
4. Continuous waves with a frequency of 15 Hz were adopted, and the current
intensity was adjusted to reduce HR by approximately 10 % as the bradycardia
threshold.
2. The left side of the neck was wiped with Alcohol.
5. The vagus nerve was stimulated by electroacupuncture for 30 min every day
for 1 week.

6. MÉTODOS
El Western Blotting tiene como finalidad identificar proteínas
presentes en una muestra basándose en una reacción Antígeno-
Anticuerpo.
Dentro del proceso de la relación del Infarto con el Nervio vago se
expresan diferentes proteínas, y para identificar cada una de ellas,
esta prueba Western Blotting es la adecuada para hacerlo.
2.10 WESTERN BLOTTING

7. Method used in the laboratory to
detect certain antigens (markers) in
a cell or tissue sample. In
immunofluorescence, antibodies
that are attached to a special dye
are used, which bind to the antigen
in the sample.
2.11 Immunofluorescence staining

METHODS

8. METHODS
It uses enzyme-linked antibodies to detect and
measure the amount of a substance in a solution,
such as serum; In the final stage, an enzymatic
reaction occurs that causes a color change that can
be read using a special machine
2.12 ELISA

9. RESULTADOS
Fig 1. Panel F
Fig 1. Panel G
Fig 2. Panel A

10. RESULTADOS
Fig 2. Panel C
Fig 3. Panel A
Fig 2. Panel G

11. Fig 4. Panel A
Fig 4. Panel B
RESULTS

12. RESULTADOS

13. DISCUSSION
AUTHOR OPINION
AGREE/DISAGREE
They reach the brain during
development, migrate along
blood vessels, and are
associated with various types of
neuroinflammation in the central
nervous system S.D Skaper, et al
J.J Hamann , et al
In addition, VNS treatment
improved left ventricular
systolic function and improved
heart failure biomarkers
compared to those in the control
group
also reported that chymase is a
major determinant of the
production of AngII in the rat
heart, and AngII itself is a
growth factor and stimulator of
proinflammatory cytokines,
such as TNF-α
S. Ahmad, et al.

14. CONCLUSIONS
• Molecular biology helped in the research
regarding the methods (with immunofluorescence,
ELISA, and western blotting) to achieve a positive
result on the VNS research in people with a history
of stroke.
• Thanks to molecular biology, it was possible to
monitor individuals who have had strokes and their
long-term effects.
• Molecular biology helps us obtain results to find a
partial or total solution; so that patients do not
have further complications and can lead a more
peaceful life.

15. ACCIDENTE
CEREBROVASCULAR
SUCEDE CUANDO
Hay dos tipos principales de
ACV, isquémico y hemorrágico
Pérdida de movimiento
muscular
1.
Problemas al tragar/hablar2.
Pérdida de la memoria 3.
Problemas para controlar
emociones
4.
Dolor/Sensación de
hormigueo en zonas
afectadas
5.
CAUSAS SÍNTOMAS
COMPLICACIONES
Se detiene el flujo de sangre en una parte del cerebro,
evitando que se reciba oxígeno en este tejido
HemorrágicoIsquémico
Se da por Se da por
Una arteria obstruida en el
cerebro
Rotura de un vaso
sanguíneo en el
cerebro
Los más frecuentes son
Dificultad para hablar y
entender lo que otros dicen
Entumecimiento de la
Cara, Brazo o pierna
Problemas para ver
en uno o ambos ojos
Dolor de cabeza
Problemas para caminar
Depende de
Cuánto tiempo está el cerebro
sin flujo sanguíneo y en donde
está localizado
MANUELA MONTOYA BUILES

16. Mapa 2
AUTOFAGIA
posible consecuencia
DESGRANULACION
DE MASTOCITOS
ATAQUE CEREBROVASCULAR
QUIMASA
DAÑO CARDIACO
las células se dañan o
se les priva de los
nutrientes que
necesitan para
sobrevivir
INFLAMACIÓN
SISTÉMICA
lo que pasa
causa
ANGIOTENSINA
II
ocurre
causa
vasoconstricción =
aumento de PA
puede causar
hipertensión
arterial
hipertrofia
disfunción
ventricular
su definición:
Sara Manuela Montoya Giraldo