sample handling training 21042022 accurate

sample

1. Specimen handling & transportation 1 PRESENTER NAYIGA JULIET Regional Hub coordinator

2. Presentation contents Sample collection and Labelling Safe handling of samples and package Sample rejection 2

3. SPECIMEN COLLECTION Safety precautions Put on protection gear all times. Change gloves to every patient. Wash hands often / Sanitize with 70% alcohol Ensure proper waste handling. Avoid recapping of needles Accident reports and prophylaxis. 3

4. Tube type used in sample collection Red Top ADDITIVE None Tests LFTS,RFTS,LP,TFTS, BAT,RF, HIV,HEP.B,SYPHILIS, CRAG TEST 4

5. Cont….. Purple Top ADDITIVE EDTA TESTS Hematology (CBC) and Blood group & Cross-match)Film report, ESR,HEP.B,SYPHILIS,HIV 5

6. Sample transportation Samples must be transported in cool boxes. Staff should be in gloves to remove samples from the cool box to the tray at the laboratory reception Carry request forms outside the cool box 6

7. Packaging of infectious materials Primary container is leak-proof Secondary container is leak-proof Outer container must be of adequate strength 7

8. Tripple packaging 8

9. Sample Rejection Criteria 1. Sample with no request form 2. Clotted samples for immunology or hematology tests 3. Haemolysed samples for chemistry, immunology and Hematology tests. 4. Sample brought in hands or wrapped in request forms 9

10. Recommendations Collection of EID PCR samples in purple top to improve on TAT Collection of sputum genexpert samples Ensure proper packaging of samples before delivering them at the HUB. Ensure proper filling of the request form to accompany a well labelled sample 10

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