Sangaragomathy IEC.pptx development of novel fucoidan

development of novel fucoidan

1. Presented by, Dr Sangaragomathy A M 1 st Year Post Graduate Student Department of Public Health Dentistry DEVELOPMENT OF NOVEL FUCOIDAN INCORPORATED NANO HYDROXYAPATITE ENAMEL ADHESIVE IN ENAMEL BIOMINERALIZATION – EX-VIVO ANALYSIS Guided by, Dr Shruthi Eshwar Professor Department of Public Health Dentistry

2. 2 CONTENTS NEED FOR THE STUDY AIM OF THE STUDY OBJECTIVES MATERIALS AND METHODS INCLUSION & EXCLUSION CRITERIA STUDY PROCEDURE STATISTICAL ANALYSIS BUDGET

3. NEED FOR THE STUDY ENAMEL is composed approximately 96% of inorganic material Susceptible to ACID – causing DEMINERALIZATION Imbalance in Demineralization & Remineralization – Results in DENTAL CARIES But can be REVERSED, If managed early using REMINERALIZING AGENTS Significant Public health issue Due to its high prevalence 3

4. 4 Nano Hydroxyapatite crystals Biocompatible & Bioactive material Structure similar to apatite crystals in enamel. Comparable alternative to   Fucoidan - Fucus vesiculosus Sulfated polysaccharide Source : Brown seaweed Structure & function similar to Heparin Smaller particle size allowing deeper penetration More homogenous remineralization compared to fluoride Inhibits bacterial plaque adhesion Anti-inflammatory Anti-bacterial Tissue regenerating properties

5. AIM & OBJECTIVES AIM To assess the efficacy of Fucoidan incorporated Nano hydroxyapatite-based enamel adhesive in enamel biomineralization OBJECTIVES Development of Fucoidan incorporated Nano hydroxy-apatite based enamel adhesive for enamel biomineralization. To evaluate the efficacy of Fucoidan incorporated Nano hydroxy-apatite based enamel adhesive for enamel remineralization. To compare & evaluate the efficacy of Fucoidan incorporated Nano hydroxy-apatite based enamel adhesive with CPP-ACP based enamel Remineralizing agent 5

6. MATERIALS AND METHODS Study Design : Experimental Study – In – Vitro analysis Duration of Study : 3 months Source of Study : In-Vitro study, Human Permanent teeth (Molars & Premolars) extracted for orthodontic reasons Sample Size : 24 with 6 samples per group 6

7. SELECTION CRITERIA Inclusion Criteria The Buccal & Lingual surface of the Extracted teeth under study should be free of : Macroscopic cracks Hypoplasia Carious lesions Exclusion Criteria The Buccal & Lingual surface of the Extracted teeth showing any presence of Visible cracks Fractures Decay 7

8. STUDY PROCEDURE Synthesis of Enamel Adhesive ↓ Physio-chemical Characterization (EDX, FITR, SEM, XRD) ↓ (In-Vitro Analysis) Sample Selection – 24 Extracted Human Permanent teeth Sample Prep : 24 enamel samples with Enamel window preparation Pre-assessment – SEM, EDX (surface roughness & mineral content) ↓ Inducing White spot lesions (Simple mineralization model) Intermediate Assessment – SEM, EDX ↓ Sample Grouping (Based on Remineralizing agent used) Group 1 Group 2 Group 3 Group 4 n = 6 n = 6 n = 6 n = 6 Fucoidan varnish Fucoidan + nHAP n HAP CPP- ACP ↓ 28-day pH cycling model (To simulate Demineralization – Remineralization) Final Assessment of specimen – SEM, EDS ↓ Statistical Analysis 8

9. STATISTICAL ANALYSIS Descriptive statistics will be analyzed using Mean and Standard Deviation. Inferential statistics will be analyzed using ANOVA/ Kruskal Wallis, and any other test will be added as needed. Data will be processed and analyzed using [SPSS] for Windows Version 22.0 Released 2013. 9

10. BUDGET Fucoidan and nano Hydroxy-apatite Materials – Rs 15,000 Physicochemical characterization - using SEM, EDX, FITR, XRD - Rs 15,000 TOTAL – Rs 30,000 Self Funded 10

11. THANK YOU 11