unit 4- infrared spectroscopy for engineering students

unit 4- infrared spectroscopy for engineering students

1. Infrared Spectroscopy

2. IR Spectroscopy Introduction Spectroscopy is the study of the interaction of matter with the electromagnetic spectrum Electromagnetic radiation displays the properties of both particles and waves The particle component is called a photon The energy ( E ) component of a photon is proportional to the frequency. Where h is Planck’s constant and n is the frequency in Hertz (cycles per second) E = h n The term “photon” is implied to mean a small, massless particle that contains a small wave-packet of EM radiation/light – we will use this terminology in the course

3. IR Spectroscopy Introduction Because the speed of light, c , is constant, the frequency, n , (number of cycles of the wave per second) can complete in the same time, must be inversely proportional to how long the oscillation is, or wavelength : Amplitude, A , describes the wave height, or strength of the oscillation Because the atomic particles in matter also exhibit wave and particle properties (though opposite in how much) EM radiation can interact with matter in two ways: Collision – particle-to-particle – energy is lost as heat and movement Coupling – the wave property of the radiation matches the wave property of the particle and “couple” to the next higher quantum mechanical energy level n = c = 3 x 10 10 cm/s ___ l c  E = h n = ___ l hc

4. IR Spectroscopy Introduction The entire electromagnetic spectrum is used by chemists: UV X-rays IR g -rays Radio Microwave Energy (kcal/mol) 300-30 300-30 ~10 -4 > 300 ~10 -6 Visible Frequency , n in Hz ~10 15 ~10 13 ~10 10 ~10 5 ~10 17 ~10 19 Wavelength , l 10 nm 1000 nm 0.01 cm 100 m ~0.01 nm ~.0001 nm nuclear excitation (PET) core electron excitation (X-ray cryst.) electronic excitation ( p to p *) molecular vibration molecular rotation Nuclear Magnetic Resonance NMR (MRI)

5. IR Spectroscopy Introduction The IR Spectroscopic Process The quantum mechanical energy levels observed in IR spectroscopy are those of molecular vibration We perceive this vibration as heat When we say a covalent bond between two atoms is of a certain length, we are citing an average because the bond behaves as if it were a vibrating spring connecting the two atoms For a simple diatomic molecule, this model is easy to visualize:

6. IR Spectroscopy Introduction The IR Spectroscopic Process There are two types of bond vibration: Stretch – Vibration or oscillation along the line of the bond Bend – Vibration or oscillation not along the line of the bond H H C H H C scissor asymmetric H H C C H H C C H H C C H H C C symmetric rock twist wag in plane out of plane

7. The IR Spectroscopic Process As a covalent bond oscillates – due to the oscillation of the dipole of the molecule – a varying electromagnetic field is produced The greater the dipole moment change through the vibration, the more intense the EM field that is generated Infrared Spectroscopy

8. The IR Spectroscopic Process When a wave of infrared light encounters this oscillating EM field generated by the oscillating dipole of the same frequency, the two waves couple, and IR light is absorbed The coupled wave now vibrates with twice the amplitude Infrared Spectroscopy IR beam from spectrometer EM oscillating wave from bond vibration “coupled” wave

9. The IR Spectrum Each stretching and bending vibration occurs with a characteristic frequency as the atoms and charges involved are different for different bonds The y-axis on an IR spectrum is in units of % transmittance In regions where the EM field of an osc. bond interacts with IR light of the same n – transmittance is low (light is absorbed) In regions where no osc. bond is interacting with IR light, transmittance nears 100% Infrared Spectroscopy

10. IR Spectroscopy The IR Spectrum The x-axis of the IR spectrum is in units of wavenumbers , n , which is the number of waves per centimeter in units of cm -1 (Remember E = h n or E = hc / l )

11. IR Spectroscopy The IR Spectrum This unit is used rather than wavelength (microns) because wavenumbers are directly proportional to the energy of transition being observed – chemists like this, physicists hate it High frequencies and high wavenumbers equate higher energy is quicker to understand than Short wavelengths equate higher energy This unit is used rather than frequency as the numbers are more “real” than the exponential units of frequency IR spectra are observed for the mid-infrared: 600-4000 cm -1 The peaks are Gaussian distributions of the average energy of a transition

12. IR Spectroscopy The IR Spectrum In general: Lighter atoms will allow the oscillation to be faster – higher energy This is especially true of bonds to hydrogen – C-H, N-H and O-H Stronger bonds will have higher energy oscillations Triple bonds > double bonds > single bonds in energy Energy/ n of oscillation

13. The IR Spectrum – The detection of different bonds As opposed to chromatography or other spectroscopic methods, the area of a IR band (or peak) is not directly proportional to concentration of the functional group producing the peak The intensity of an IR band is affected by two primary factors: Whether the vibration is one of stretching or bending Electronegativity difference of the atoms involved in the bond For both effects, the greater the change in dipole moment in a given vibration or bend, the larger the peak. The greater the difference in electronegativity between the atoms involved in bonding, the larger the dipole moment Typically, stretching will change dipole moment more than bending Infrared Spectroscopy

14. The IR Spectrum – The detection of different bonds It is important to make note of peak intensities to show the effect of these factors: Strong (s) – peak is tall, transmittance is low (0-35 %) Medium (m) – peak is mid-height (75-35%) Weak (w) – peak is short, transmittance is high (90-75%) * Broad ( br ) – if the Gaussian distribution is abnormally broad (*this is more for describing a bond that spans many energies) Exact transmittance values are rarely recorded Infrared Spectroscopy

15. II. Infrared Group Analysis A. General The primary use of the IR is to detect functional groups Because the IR looks at the interaction of the EM spectrum with actual bonds, it provides a unique qualitative probe into the functionality of a molecule, as functional groups are merely different configurations of different types of bonds Since most “types” of bonds in covalent molecules have roughly the same energy, i.e., C=C and C=O bonds, C-H and N-H bonds they show up in similar regions of the IR spectrum Remember all organic functional groups are made of multiple bonds and therefore show up as multiple IR bands (peaks) Infrared Spectroscopy

16. II. Infrared Group Analysis A. General The four primary regions of the IR spectrum 4000 cm -1 2700 cm -1 2000 cm -1 1600 cm -1 600 cm -1 O-H N-H C-H C≡C C≡N C=O C=N C=C Fingerprint Region C-C C-N C-O Infrared Spectroscopy Bonds to H Triple bonds Double bonds Single Bonds

17. Alkanes – combination of C-C and C-H bonds C-C stretches and bends 1360-1470 cm -1 CH 2 -CH 2 bond 1450-1470 cm -1 CH 2 -CH 3 bond 1360-1390 cm -1 sp 3 C-H between 2800-3000 cm -1 Infrared Spectroscopy Octane (w – s) (m)

18. Alkenes – addition of the C=C and vinyl C-H bonds C=C stretch at 1620-1680 cm -1 weaker as substitution increases vinyl C-H stretch occurs at 3000-3100 cm -1 The difference between alkane , alkene or alkyne C-H is important! If the band is slightly above 3000 it is vinyl sp 2 C-H or alkynyl sp C-H if it is below it is alkyl sp 3 C-H 1-Octene Infrared Spectroscopy (w – m) (w – m)

19. Alkynes – addition of the C=C and vinyl C-H bonds C≡C stretch 2100-2260 cm -1 ; strength depends on asymmetry of bond, strongest for terminal alkynes, weakest for symmetrical internal alkynes C-H for terminal alkynes occurs at 3200-3300 cm -1 Internal alkynes ( R-C ≡ C-R ) would not have this band! 1-Octyne Infrared Spectroscopy (m – s) (w-m)

20. Aromatics Due to the delocalization of e - in the ring, C-C bond order is 1.5, the stretching frequency for these bonds is slightly lower in energy than normal C=C These show up as a pair of sharp bands, 1500 & 1600 cm -1 , (lower frequency band is stronger) C-H bonds off the ring show up similar to vinyl C-H at 3000-3100 cm -1 Ethyl benzene Infrared Spectroscopy (w – m) (w – m)

21. Aromatics If the region between 1667-2000 cm -1 (w) is free of interference (C=O stretching frequency is in this region) a weak grouping of peaks is observed for aromatic systems Analysis of this region, called the overtone of bending region, can lead to a determination of the substitution pattern on the aromatic ring Monosubstituted 1,2 disubstituted ( ortho or o -) 1,2 disubstituted ( meta or m -) 1,4 disubstituted ( para or p -) Infrared Spectroscopy

22. Unsaturated Systems – substitution patterns The substitution of aromatics and alkenes can also be discerned through the out-of-plane bending vibration region However, other peaks often are apparent in this region. These peaks should only be used for reinforcement of what is known or for hypothesizing as to the functional pattern. Infrared Spectroscopy

23. Ethers – addition of the C-O-C asymmetric band and vinyl C-H bonds Show a strong band for the antisymmetric C-O-C stretch at 1050-1150 cm -1 Otherwise, dominated by the hydrocarbon component of the rest of the molecule Diisopropyl ether Infrared Spectroscopy (s)

24. Alcohols Strong , broad O-H stretch from 3200-3400 cm -1 Like ethers, C-O stretch from 1050-1260 cm -1 Band position changes depending on the alcohols substitution: 1 ° 1075-1000; 2 ° 1075-1150; 3 ° 1100-1200; phenol 1180-1260 The shape is due to the presence of hydrogen bonding 1-butanol Infrared Spectroscopy (m– s) br (s)

25. Amines - Primary Shows the –N-H stretch for NH 2 as a doublet between 3200-3500 cm -1 (symmetric and anti-symmetric modes) -NH 2 has deformation band from 1590-1650 cm -1 Additionally there is a “wag” band at 780-820 cm -1 that is not diagnostic 2-aminopentane Infrared Spectroscopy (w) (w)

26. Amines – Secondary N-H band for R 2 N-H occurs at 3200-3500 cm -1 as a single sharp peak weaker than –O-H Tertiary amines (R 3 N) have no N-H bond and will not have a band in this region pyrrolidine Infrared Spectroscopy (w – m)

27. Infrared Spectroscopy Pause and Review Inspect the bonds to H region (2700 – 4000 cm -1 ) Peaks from 2850-3000 are simply sp 3 C-H in most organic molecules Above 3000 cm -1 Learn shapes, not wavenumbers ! : Broad U-shape peak - O—H bond V-shape peak -N—H bond for 2 o amine (R 2 N—H ) Sharp spike -C ≡ C—H bond W-shape peak -N—H bond for 1 o amine (R NH 2 ) 3000 cm -1 Small peak shouldered just above 3000 cm -1 C= C—H or Ph —H

28. Aldehydes C=O (carbonyl) stretch from 1720-1740 cm -1 Band is sensitive to conjugation, as are all carbonyls (upcoming slide ) A highly unique sp 2 C-H stretch appears as a doublet, 2720 & 2820 cm -1 called a “ Fermi doublet ” Cyclohexyl carboxaldehyde Infrared Spectroscopy (w-m) (s)

29. Ketones Simplest of the carbonyl compounds as far as IR spectrum – carbonyl only C=O stretch occurs at 1705-1725 cm -1 3-methyl-2-pentanone Infrared Spectroscopy (s)

30. Esters C=O stretch at 1735-1750 cm -1 Strong band for C-O at a higher frequency than ethers or alcohols at 1150-1250 cm -1 Infrared Spectroscopy Ethyl pivalate (s) (s)

31. Carboxylic Acids: Gives the messiest of IR spectra C=O band occurs between 1700-1725 cm -1 The highly dissociated O-H bond has a broad band from 2400-3500 cm -1 covering up to half the IR spectrum in some cases 4-phenylbutyric acid Infrared Spectroscopy (w – m) br (s) (s)

32. Acid anhydrides Coupling of the anhydride though the ether oxygen splits the carbonyl band into two with a separation of 70 cm -1 Bands are at 1740-1770 cm-1 and 1810-1840 cm -1 Mixed mode C-O stretch at 1000-1100 cm -1 Propionic anhydride Infrared Spectroscopy (s) (s)

33. Acid halides Clefted band at 1770-1820 cm -1 for C=O Bonds to halogens, due to their size (see Hooke’s Law derivation) occur at low frequencies, only Cl is light enough to have a band on IR, C- Cl is at 600-800 cm -1 Propionyl chloride Infrared Spectroscopy (s) (s)

34. Amides Display features of amines and carbonyl compounds C=O stretch at 1640-1680 cm -1 If the amide is primary (-NH 2 ) the N-H stretch occurs from 3200-3500 cm -1 as a doublet If the amide is secondary (-NHR) the N-H stretch occurs at 3200-3500 cm -1 as a sharp singlet pivalamide Infrared Spectroscopy (m – s) (s)

35. Nitro group (-NO 2 ) Proper Lewis structure gives a bond order of 1.5 from nitrogen to each oxygen Two bands are seen (symmetric and asymmetric) at 1300-1380 cm -1 and 1500-1570 cm -1 This group is a strong resonance withdrawing group and is itself vulnerable to resonance effects 2-nitropropane Infrared Spectroscopy (s) (s)

36. Nitriles (the cyano - or –C ≡ N group) Principle group is the carbon nitrogen triple bond at 2100-2280 cm -1 This peak is usually much more intense than that of the alkyne due to the electronegativity difference between carbon and nitrogen propionitrile Infrared Spectroscopy (s)

37. Effects on IR bands Conjugation – by resonance, conjugation lowers the energy of a double or triple bond. The effect of this is readily observed in the IR spectrum: Conjugation will lower the observed IR band for a carbonyl from 20-40 cm -1 provided conjugation gives a strong resonance contributor Inductive effects are usually small, unless coupled with a resonance contributor (note –CH 3 and – Cl above) Infrared Spectroscopy

38. Effects on IR bands Steric effects – usually not important in IR spectroscopy, unless they reduce the strength of a bond (usually p ) by interfering with proper orbital overlap: Here the methyl group in the structure at the right causes the carbonyl group to be slightly out of plane, interfering with resonance Strain effects – changes in bond angle forced by the constraints of a ring will cause a slight change in hybridization, and therefore, bond strength As bond angle decreases, carbon becomes more electronegative, as well as less sp 2 hybridized (bond angle < 120 ° ) Infrared Spectroscopy

39. Effects on IR bands Hydrogen bonding Hydrogen bonding causes a broadening in the band due to the creation of a continuum of bond energies associated with it In the solution phase these effects are readily apparent; in the gas phase where these effects disappear or in lieu of steric effects, the band appears as sharp as all other IR bands: Gas phase spectrum of 1-butanol Steric hindrance to H-bonding in a di- tert -butylphenol H-bonding can interact with other functional groups to lower frequencies Infrared Spectroscopy