Salivary Amylase And Phosphorylase
Introduction
The purpose of this experiment was to determine the enzyme activity for salivary amylase and
phosphorylase by placing them under different conditions of temperature and enzyme concentration.
An enzyme is a protein that acts as a catalyst to form reaction, and see the activity of the specific
enzyme (Knowles, 1991). One part of the enzyme, salivary amylase, is that alpha amylase is in the
saliva of most animals because this enzyme breaks down starch (Jacobsen, Melvaer, Hensten
Pettersen, 1972). In the presence of starch, this enzyme is present in saliva, but is not present when
there is no starch present (Jacobsen, Melvaer, Hensten Pettersen, 1972). The conditions for salivary
amylase to have a reaction with starch would change in temperature and enzyme concentration, as
well as, monitoring the pH levels (Jacobsen, Melvaer, Hensten Pettersen, 1972). Salivary amylase is
an enzyme is human saliva that helps in digestion of specific substrates, such as starch (Hudman,
Friend, Hartman, Ashton, Catron, 1957). It breaks down starch molecules by splitting maltose from
the non reducing end of a gluten molecule (Jacobsen, Melvaer, Hensten Pettersen, 1972).
Phosphorylase acts on starch and glycogen by breaking it down by breaking the glucose bonds and
taking a glucose molecule off of the structure (Ball, Vialle, Alonso Casajus, Duavillee, Munoz, Baroja
Fernandez, Moran Zorzano, Eydallin, Pozueta Romero, 2006). The conditions for the reaction of
Phosphorylase and
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